• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZHANG Ying, WANG Di, ZHANG Pei, ZHANG Zunjian, XU Fengguo. Metabolomic study on the effects of insulin and oleic acid on the development of colon cancer xenografts[J]. Journal of China Pharmaceutical University, 2021, 52(3): 339-345. DOI: 10.11665/j.issn.1000-5048.20210311
Citation: ZHANG Ying, WANG Di, ZHANG Pei, ZHANG Zunjian, XU Fengguo. Metabolomic study on the effects of insulin and oleic acid on the development of colon cancer xenografts[J]. Journal of China Pharmaceutical University, 2021, 52(3): 339-345. DOI: 10.11665/j.issn.1000-5048.20210311
shu

Metabolomic study on the effects of insulin and oleic acid on the development of colon cancer xenografts

  • Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing 210009, China

Funds: This study was supported by the National Natural Science Foundation of China (No.81773682, No.81773861) and Jiangsu Provincial National Science Foundation for Distinguished Young Scholars (No. BK20180027)
More Information
  • Received Date: March 07, 2021
  • Revised Date: March 21, 2021
    Graphical Abstract
    • Abstract
  • To investigate the regulatory effects of insulin and oleic acid on serum metabolites in colon cancer, subcutaneous transplantation tumor model of colon carcinoma cell HCT116 was established. Nude mice were randomly divided into 4 groups: control (CON, vehicle); insulin treatment (INS, sc, 2.5 U/kg); oleic acid treatment (OA, ig, 2.0 g/kg); and insulin (sc, 2.5 U/kg) plus oleic acid (ig, 2.0 g/kg) treatment (IO). Non-target metabolomic analysis on the blood samples was performed by GC/MS and LC-IT-TOF/MS. Data pre-processing, including peaking, spectral deconvolution and peak alignment, was performed before data were imported to SIMCA-P for multivariate statistical analysis. Results showed that body weight of individuals in IO group was the lowest, but the tumor weight was the heaviest. Metabolic profiles of IO group were also different compared with the CON group, and the contributing metabolites were urea, arabinose, cholesterol, L-acetylcarnitine and sphingosine. There was no significant difference between OA or INS and CON. This study showed that the combination of insulin and oleic acid promoted colon cancer deterioration and caused metabolic disturbance in blood.Our study may provide theoretical foundation for the discovery of colon cancer biomarker and its early diagnosis.
    • FullText(HTML)
    • References (18)
  • [1]
    . CA Cancer J Clin， 2020， 70（3）： 145-164.
    [2]
    Liu XD， Hemminki K， F?rsti A， et al. Cancer risk in patients with type 2 diabetes mellitus and their relatives［J］. Int J Cancer， 2015， 137（4）： 903-910.
    [3]
    Frezza EE， Wachtel MS， Chiriva-Internati M. Influence of obesity on the risk of developing colon cancer［J］. Gut， 2006， 55（2）： 285-291.
    [4]
    Chen X， Liang H， Song Q， et al. Insulin promotes progression of colon cancer by upregulation of ACAT1［J］. Lipids Health Dis， 2018， 17（1）： 122.
    [5]
    Choi S， Yoo YJ， Kim H， et al. Clinical and biochemical relevance of monounsaturated fatty acid metabolism targeting strategy for cancer stem cell elimination in colon cancer［J］. Biochem Biophys Res Commun， 2019， 519（1）： 100-105.
    [6]
    Soto-Guzman A， Navarro-Tito N， Castro-Sanchez L， et al. Oleic acid promotes MMP-9 secretion and invasion in breast cancer cells［J］. Clin Exp Metastasis， 2010， 27（7）： 505-515.
    [7]
    Vinciguerra M， Carrozzino F， Peyrou M， et al. Unsaturated fatty acids promote hepatoma proliferation and progression through downregulation of the tumor suppressor PTEN［J］. J Hepatol， 2009， 50（6）： 1132-1141.
    [8]
    Xu HJ， Zhou S， Tang QL， et al. Cholesterol metabolism： New functions and therapeutic approaches in cancer［J］. Biochim Biophys Acta Rev Cancer， 2020， 1874（1）： 188394.
    [9]
    Lucenteforte E， Talamini R， Montella M， et al. Macronutrients， fatty acids and cholesterol intake and endometrial cancer［J］. Ann Oncol， 2008， 19（1）： 168-172.
    [10]
    Wang Y， Liu C， Hu L. Cholesterol regulates cell proliferation and apoptosis of colorectal cancer by modulating miR-33a-PIM3 pathway［J］. Biochem Biophys Res Commun， 2019， 511（3）： 685-692.
    [11]
    Raza S， Meyer M， Goodyear C， et al. The cholesterol metabolite 27-hydroxycholesterol stimulates cell proliferation via ERβ in prostate cancer cells［J］. Cancer Cell Int， 2017， 17： 52.
    [12]
    Kim S， Lee M， Dhanasekaran DN， et al. Activation of LXRɑ/β by cholesterol in malignant ascites promotes chemoresistance in ovarian cancer［J］. BMC Cancer， 2018， 18（1）： 1232.
    [13]
    Sharma B， Agnihotri N. Role of cholesterol homeostasis and its efflux pathways in cancer progression［J］. J Steroid Biochem Mol Biol， 2019， 191： 105377.
    [14]
    El Imrani I， Dionne S， Saragosti D， et al. Dietary supplementation of carnitine and acetylcarnitine reduce the severity of DSS colitis associated colon cancer in mice［J］. Gastroenterology， 2011， 140（5）： S-82.
    [15]
    Elimrani I， Dionne S， Saragosti D， et al. Acetylcarnitine potentiates the anticarcinogenic effects of butyrate on SW480 colon cancer cells［J］. Int J Oncol， 2015， 47（2）： 755-763.
    [16]
    Baci D， Bruno A， Bassani B， et al. Acetyl-l-carnitine is an anti-angiogenic agent targeting the VEGFR2 and CXCR4 pathways［J］. Cancer Lett， 2018， 429： 100-116.
    [17]
    Soltani G， Poursheikhani A， Yassi M， et al. Obesity， diabetes and the risk of colorectal adenoma and cancer［J］. BMC Endocr Disord， 2019， 19（1）： 113.
    [18]
    Rodriguez-Monterrosas C， Diaz-Aragon R， Cortes-Reynosa P， et al. Linoleic acid induces an increased response to insulin in MDA-MB-231 breast cancer cells［J］. J Cell Biochem， 2018， 119（7）： 5413-5425.
    • Related Articles

  • Related Articles

    [1]LI Juanjuan, HUANG Yue, WANG Yahe, ZHANG Lianxiang, QIANG Yuanyuan, GUO Le, LIU Kunmei. Antiepileptic and neuroprotective mechanism of ursolic acid based on full-length transcriptome analysis[J]. Journal of China Pharmaceutical University, 2024, 55(4): 512-521. DOI: 10.11665/j.issn.1000-5048.2023111303
    [2]PEI Ting, WANG Yuying. Synthesis and preliminary anti-tumor activity of novel ursolic acid derivative-chalcone conjugates[J]. Journal of China Pharmaceutical University, 2017, 48(1): 31-41. DOI: 10.11665/j.issn.1000-5048.20170105
    [3]CHEN Hong, YANG Jie, CUI Wei-xi, WANG Qiang. Effects and mechanism of ursolic acid on lipopolysaccharide-induced THP-1 cells[J]. Journal of China Pharmaceutical University, 2011, 42(5): 447-451.
    [4]WANG Jing-song, SHEN Jing, ZHANG Ting, TANG Cong, REN Tian-nian, XI Tao. Ursolic acid downregulates COX-2 expression by suppressing the activation of ERK in A549 cells[J]. Journal of China Pharmaceutical University, 2011, 42(1): 68-72.
    [5]Inhibitory effects and mechanism of ursolic acid on the proliferation of rataortic smooth muscle cells[J]. Journal of China Pharmaceutical University, 2010, 41(1): 66-69.
    [6]ZHENG Kai-bo, SUN Cheng-bin, MAO Hai-li, YANG Zai-bo. Progress in the research of chemical structural modification of ursolic acid and structure-activity relationship[J]. Journal of China Pharmaceutical University, 2009, 40(6): 580-584.
    [7]Release and Identification of Monocrotalic Acid from Dehydromonocrotaline[J]. Journal of China Pharmaceutical University, 2003, (6): 18-21.
    [8]Cloning of Spinach Glycolate Oxidase cDNA and Its Construction into Yeast Expression Vector[J]. Journal of China Pharmaceutical University, 2003, (1): 83-86.
    [9]Cloning of S-adenosyl-L-methionine Synthetase Gene from Saccharomyces ceverisiae and its Expression in E.coli[J]. Journal of China Pharmaceutical University, 2002, (3): 87-89.
    [10]Determination of Four Acids Components in Keyoulin Liquid Using Derivatization by GC[J]. Journal of China Pharmaceutical University, 1998, (6): 453-455.

  • Cited by

    Periodical cited type(1)

    1. 朱灵英，郭娟，张爱丽，王宝婕，曾礼芳，徐福荣，马晓惠. 参与植物三萜生物合成的细胞色素P450酶研究进展. 中草药. 2019(22): 5597-5610 .

    Other cited types(3)

Catalog

    Get Citation
    PDF
    XML
    Article views (194) PDF downloads (696) Cited by(4)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：318963

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return