Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ_25-35

In this study, the effects and mechanisms of miR-23b in the AD cell model were explored. Aβ_25-35 was used to induce neuronal injury model, and cell viabilities were detected by MTT assay. The effect of miR-23b on the apoptotic levels of Aβ_25-35-induced SH-SY5Y cells was analyzed using Annexin V-FITC/PI detection kit. The effect of miR-23b on the mitochondrial membrane potential of Aβ_25-35-induced SH-SY5Y cells was examined using JC-1 fluorescent probe. The levels of cell apoptosis-related proteins and autophagy-related proteins were detected by Western blot. The results showed that miR-23b could alleviate the apoptosis and the abnormal mitochondrial membrane potential in SH-SY5Y cells induced by Aβ_25-35.This study suggested that miR-23b may attenuate Aβ_25-35-induced neuronal apoptosis by regulating apoptosis and autophagy-related pathway.