• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
WANG Lixin, JIANG Xiubo, GUO Qiaozhen, WANG Zicheng, WANG Bo, WANG Yuxia, QU Wensheng, DUAN Xiaotao. TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231[J]. Journal of China Pharmaceutical University, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
Citation: WANG Lixin, JIANG Xiubo, GUO Qiaozhen, WANG Zicheng, WANG Bo, WANG Yuxia, QU Wensheng, DUAN Xiaotao. TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231[J]. Journal of China Pharmaceutical University, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514
shu

TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231

  • 1.

    School of Chemical Engineering,Tianjin University,Tianjin 300350

  • 2.

    State Key Laboratory of Antitoxic Drugs and Toxicology,Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850

  • 3.

    College of Biological and Pharmaceutical Engineering, Nanjing Tech University,Nanjing, 211800, China

More Information
  • Received Date: February 22, 2021
  • Revised Date: May 09, 2021
    Graphical Abstract
    • Abstract
  • The aim of this study was to investigate the effect of transmembrane 9 superfamily protein member 2 (TM9SF2) in proliferation and migration of triple negative breast cancer cell line MDA-MB-231.The expression of TM9SF2 in triple negative breast cancer cell line MDA-MB-231 and nontumorigenic mammary epithelial cell line MCF-10A were measured by Western blot. MDA-MB-231 cells were treated with siRNA-TM9SF2 to knock-down the expression of TM9SF2. The effect of silencing TM9SF2 was measured with Western blot.The proliferation of cells was tested by MTS,and the migration was measured with Transwell and wound-healing assay.Proteins related to proliferation (PI3K,AKT,SRC and ERK) and migration (Snail,Slug and N-cadherin) were measured with Western blot.Protein expressions of TM9SF2 was better improved in triple negative breast cancer MDA-MB-231 cell line than MCF-10A.Compared with the control group, the siRNA-TM9SF2 infected group had lower expressions of PI3K, Snail, Slug and N-cadherin, and at the same time phosphorylation of AKT was decreased. The results suggest TM9SF2 can promote the proliferation and metastasis of triple negative breast cancer MDA-MB-231 cell line.
    • FullText(HTML)
    • References (16)
  • [1]
    ？Trends Cancer，2017，3（11）：753-760.
    [2]
    Perrin J，Mortier M，Jacomin AC，et al. The nonaspanins TM9SF2 and TM9SF4 regulate the plasma membrane localization and signalling activity of the peptidoglycan recognition protein PGRP-LC in Drosophila［J］. Innate Immunity，2015，7（1）：37-46.
    [3]
    Froquet R，Cherix N，Birke R，et al. Control of cellular physiology by TM9 proteins in yeast and dictyostelium［J］. J Biol Chem，2008，283（11）：6764-6762.
    [4]
    Cornillon S，Pech E，Benghezal M，et al. Phg1p is a nine-transmembrane protein superfamily member involved in dictyostelium adhesion and phagocytosis［J］. J Biol Chem，2000，275：34287-34292.
    [5]
    Lozupone F，Perdicchio M，Brambilla D，et al. The human homologue of dictyostelium discoideum phg1A is expressed by human metastatic melanoma cells［J］. EMBO Rep，2009，10：1348-1354.
    [6]
    He P，Peng Z，Luo Y，et al. High-throughput functional screening for autophagy-related genes and identification of TM9SF1 as an autophagosome-inducing gene［J］. Autophagy，2009，5： 52-60.
    [7]
    Zaravinos A，Lambrou GI，Boulalas I. Identification of common differentially expressed genes in urinary bladder cancer［J］. PLoS One，2011，6：18135.
    [8]
    Fais S，Fauvarque M. TM9 and cannibalism： how to learn more about cancer by studying amoebae and invertebrates［J］. Trends Mol Med，2012，18（1）：4-5.
    [9]
    Sun L，Meng Z.，Zhu Y，et al. TM9SF4 is a novel factor promoting autophagic flux under amino acid starvation［J］. Cell Death Differ，2018，25（2）：368-379.
    [10]
    Zhu Y，Xie M，Meng Z，et al. Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells［J］. Oncogene，2019，38（29）：5778-5791.
    [11]
    Samah AS，Yuh S，Tuoya DY，et al. Identification of TM9SF2 as a candidate of the cell surface marker common to breast carcinoma cells［J］. Clin Oncol Cancer Res，2009，6：1-9.
    [12]
    Clark CR，Maile M，Blaney P，et al. Transposon mutagenesis screen in mice identifies TM9SF2 as a novel colorectal cancer oncogene［J］. Sci Rep，2018，8（1）：153-157.
    [13]
    Li Q，Lei CB，Lu C，et al. LINC01232 exerts oncogenic activities in pancreatic adenocarcinoma via regulation of TM9SF2［J］. Cell Death Dis，2019，10（10）：608-612.
    [14]
    Curtis C，Shah SP，Chin SF，et al. The genomic and transcriptomic architecture of 2000 breast tumours reveals novel subgroups［J］. Nature，2012，486（7403）：346-352.
    [15]
    Fruman DA，Chiu H，Hopkins BD，et al. The PI3K pathway in human disease［J］.Cell，2017，170（4）：605-635.
    [16]
    Engelman JA，Luo J，Cantley LC. The evolution of phosphatidylinositol 3-kinases as regulators of growth and metabolism［J］. Nat Rev Genet，2006，7（8）：606-610.
    • Related Articles

  • Related Articles

    [1]WANG Liming, JIANG Xiaomeng, GAO Yue, MA Yuxiao, ZENG Aizhong, GUO Wei. rhIL23R-CHR/Fc fusion protein inhibits Act-HaCaT inflammation and proliferation by downregulating ENST00000522718[J]. Journal of China Pharmaceutical University, 2022, 53(6): 734-741. DOI: 10.11665/j.issn.1000-5048.20220613
    [2]SHI Zhengke, CHEN Peipei, ZHANG Yixuan, WEI Jie, DU Yanan, LIU Xiaoquan. Up-regulated miR-452-5p suppressed the expression of RORα and promoted the proliferation and migration of HCC cells[J]. Journal of China Pharmaceutical University, 2022, 53(3): 323-332. DOI: 10.11665/j.issn.1000-5048.20220310
    [3]LIN Aihua, LIU Jin, CHEN Yanqing, ZHANG Yan, YU Yang. Effect of serum amyloid A on microglial migration and its mechanism[J]. Journal of China Pharmaceutical University, 2020, 51(5): 591-598. DOI: 10.11665/j.issn.1000-5048.20200511
    [4]DUAN Lanlan, DONG Jing, FAN Xiangcheng, ZHU Junyi, ZHANG Yifan, HAN Jichun, SHANG Jing. β-Elemene improves endothelial cells dysfunction, and abnormal proliferation and migration of vascular smooth muscle cells[J]. Journal of China Pharmaceutical University, 2020, 51(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20200311
    [5]WU You, TANG Tingting, ZHU Qinhua, JIN Liang, PAN Yi. Mechanisms of miR-29a in migration and invasion of breast cancer MCF-7 cells in vitro[J]. Journal of China Pharmaceutical University, 2018, 49(3): 348-353. DOI: 10.11665/j.issn.1000-5048.20180314
    [6]XIANG Min, ZHANG Yaqin, WU Pingping, GAO Zhenyu. Effect of block of AGEs-RAGE pathway on the migration of VSMCs[J]. Journal of China Pharmaceutical University, 2016, 47(2): 199-203. DOI: 10.11665/j.issn.1000-5048.20160212
    [7]HE Zhenhui, HE Taiping, WENG Shanfan, HUANG Yuequn, QIN Yanmei, Liang Nianci. Inhibitory effect of the diterperoid compound 5F isolated from Pteris semipinnata L.on breast cancer growth and metastasis in vivo[J]. Journal of China Pharmaceutical University, 2014, 45(1): 92-96. DOI: 10.11665/j.issn.1000-5048.20140117
    [8]MU Rong, LIU Jin-wen, HUA Fang. Construction of cell model with long-term silencing of TRB3 and TRB3 promoter reporter gene vector[J]. Journal of China Pharmaceutical University, 2012, 43(2): 182-186.
    [9]XU Chang-liang, XI Tao. Anti-migration and anti-angiogenic effects of tanshinone IIA on breast cancer cell MDA-MB-435[J]. Journal of China Pharmaceutical University, 2009, 40(6): 565-570.
    [10]WANG Wei, YAO Jing, ZHOU Jian-ping, LI Yi-ping, TAO Lei. Effects of urocanic acid-modified chitosan-mediated wild-type p53 gene transfection on proliferation and apoptosis of human lung adenocarcinoma cell line H1299[J]. Journal of China Pharmaceutical University, 2009, 40(5): 440-444.

  • Cited by

    Periodical cited type(1)

    1. 陈腾腾，陈迪. 环状RNAs-000911对三阴性乳腺癌细胞株活性的影响. 现代养生. 2023(12): 881-884 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (113) PDF downloads (563) Cited by(1)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：414982

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return