TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231

WANG Lixin; JIANG Xiubo; GUO Qiaozhen; WANG Zicheng; WANG Bo; WANG Yuxia; QU Wensheng; DUAN Xiaotao

doi:10.11665/j.issn.1000-5048.20210514

Journal of China Pharmaceutical University > 2021 > 52(5): 609-613. > DOI: 10.11665/j.issn.1000-5048.20210514

WANG Lixin, JIANG Xiubo, GUO Qiaozhen, WANG Zicheng, WANG Bo, WANG Yuxia, QU Wensheng, DUAN Xiaotao. TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231[J]. Journal of China Pharmaceutical University, 2021, 52(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20210514

Citation:

PDF (1960 KB)

TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231

1.
School of Chemical Engineering,Tianjin University,Tianjin 300350
2.
State Key Laboratory of Antitoxic Drugs and Toxicology,Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850
3.
College of Biological and Pharmaceutical Engineering, Nanjing Tech University,Nanjing, 211800, China

More Information

Received Date: February 22, 2021
Revised Date: May 09, 2021

Graphical Abstract

Abstract

Abstract

The aim of this study was to investigate the effect of transmembrane 9 superfamily protein member 2 (TM9SF2) in proliferation and migration of triple negative breast cancer cell line MDA-MB-231.The expression of TM9SF2 in triple negative breast cancer cell line MDA-MB-231 and nontumorigenic mammary epithelial cell line MCF-10A were measured by Western blot. MDA-MB-231 cells were treated with siRNA-TM9SF2 to knock-down the expression of TM9SF2. The effect of silencing TM9SF2 was measured with Western blot.The proliferation of cells was tested by MTS,and the migration was measured with Transwell and wound-healing assay.Proteins related to proliferation (PI3K,AKT,SRC and ERK) and migration (Snail,Slug and N-cadherin) were measured with Western blot.Protein expressions of TM9SF2 was better improved in triple negative breast cancer MDA-MB-231 cell line than MCF-10A.Compared with the control group, the siRNA-TM9SF2 infected group had lower expressions of PI3K, Snail, Slug and N-cadherin, and at the same time phosphorylation of AKT was decreased. The results suggest TM9SF2 can promote the proliferation and metastasis of triple negative breast cancer MDA-MB-231 cell line.
- TM9SF2,
- MDA-MB-231,
- gene silence,
- proliferation,
- migration

FullText(HTML)

References (16)

References

[1]	？Trends Cancer，2017，3（11）：753-760.
[2]	Perrin J，Mortier M，Jacomin AC，et al. The nonaspanins TM9SF2 and TM9SF4 regulate the plasma membrane localization and signalling activity of the peptidoglycan recognition protein PGRP-LC in Drosophila［J］. Innate Immunity，2015，7（1）：37-46.
[3]	Froquet R，Cherix N，Birke R，et al. Control of cellular physiology by TM9 proteins in yeast and dictyostelium［J］. J Biol Chem，2008，283（11）：6764-6762.
[4]	Cornillon S，Pech E，Benghezal M，et al. Phg1p is a nine-transmembrane protein superfamily member involved in dictyostelium adhesion and phagocytosis［J］. J Biol Chem，2000，275：34287-34292.
[5]	Lozupone F，Perdicchio M，Brambilla D，et al. The human homologue of dictyostelium discoideum phg1A is expressed by human metastatic melanoma cells［J］. EMBO Rep，2009，10：1348-1354.
[6]	He P，Peng Z，Luo Y，et al. High-throughput functional screening for autophagy-related genes and identification of TM9SF1 as an autophagosome-inducing gene［J］. Autophagy，2009，5： 52-60.
[7]	Zaravinos A，Lambrou GI，Boulalas I. Identification of common differentially expressed genes in urinary bladder cancer［J］. PLoS One，2011，6：18135.
[8]	Fais S，Fauvarque M. TM9 and cannibalism： how to learn more about cancer by studying amoebae and invertebrates［J］. Trends Mol Med，2012，18（1）：4-5.
[9]	Sun L，Meng Z.，Zhu Y，et al. TM9SF4 is a novel factor promoting autophagic flux under amino acid starvation［J］. Cell Death Differ，2018，25（2）：368-379.
[10]	Zhu Y，Xie M，Meng Z，et al. Knockdown of TM9SF4 boosts ER stress to trigger cell death of chemoresistant breast cancer cells［J］. Oncogene，2019，38（29）：5778-5791.
[11]	Samah AS，Yuh S，Tuoya DY，et al. Identification of TM9SF2 as a candidate of the cell surface marker common to breast carcinoma cells［J］. Clin Oncol Cancer Res，2009，6：1-9.
[12]	Clark CR，Maile M，Blaney P，et al. Transposon mutagenesis screen in mice identifies TM9SF2 as a novel colorectal cancer oncogene［J］. Sci Rep，2018，8（1）：153-157.
[13]	Li Q，Lei CB，Lu C，et al. LINC01232 exerts oncogenic activities in pancreatic adenocarcinoma via regulation of TM9SF2［J］. Cell Death Dis，2019，10（10）：608-612.
[14]	Curtis C，Shah SP，Chin SF，et al. The genomic and transcriptomic architecture of 2000 breast tumours reveals novel subgroups［J］. Nature，2012，486（7403）：346-352.
[15]	Fruman DA，Chiu H，Hopkins BD，et al. The PI3K pathway in human disease［J］.Cell，2017，170（4）：605-635.
[16]	Engelman JA，Luo J，Cantley LC. The evolution of phosphatidylinositol 3-kinases as regulators of growth and metabolism［J］. Nat Rev Genet，2006，7（8）：606-610.

Cited By

Get Citation

PDF

XML

Article views (113) PDF downloads (562)

Turn off MathJax

Article Contents

Abstract

References

TM9SF2 promotes proliferation and metastasis of triple negative breast cancer cell line MDA-MB-231

Abstract

References

Catalog

Export File

Citation

Format

Content