Protective effects and mechanism of icariin against vascular function in diabetic mice

WANG Xiniao; YAO Wenhui; PAN Zhenzhen; DONG Jieyan; LIU Shuo; DING Xuansheng

doi:10.11665/j.issn.1000-5048.20220212

Journal of China Pharmaceutical University > 2022 > 53(2): 215-221. > DOI: 10.11665/j.issn.1000-5048.20220212

WANG Xiniao, YAO Wenhui, PAN Zhenzhen, DONG Jieyan, LIU Shuo, DING Xuansheng. Protective effects and mechanism of icariin against vascular function in diabetic mice[J]. Journal of China Pharmaceutical University, 2022, 53(2): 215-221. DOI: 10.11665/j.issn.1000-5048.20220212

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Protective effects and mechanism of icariin against vascular function in diabetic mice

School of Basic Medicine and Clinical Pharmacy, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Science and Technology Major Project-Major New Drug Innovation Foundation (No.2017ZX09301004) and the National Natural Science Foundation of China (No.81873131)

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Received Date: October 17, 2021
Revised Date: March 08, 2022

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Abstract

To explore the effects and molecular mechanism of icariin on the vascular function of mice with type 1 diabetes induced by alloxan, type 1 diabetic mice model was established by intraperitoneal injection with 200 mg/kg alloxan.After oral administration with icariin (60, 120 mg/kg) daily for 2 weeks, blood glucose, body weight, food intake and water intake were detected.To evaluate the impact of icariin on the function of isolated vascular ring contraction and relaxation, thoracic aortas of mice were removed and the Ach-induced vascular ring relaxation, Phe-induced vascular ring contraction, SNP-induced vascular ring relaxation and KCl-induced vascular ring contraction response were detected.To further confirm the mechanism of icariin to improve vascular function, human umbilical vein endothelial cells (HUVECs) were induced by high glucose (HG) in vitro.Western blot was used to detect the effect of icariin on eNOS, p-eNOS, p38 MAPK and p-p38 MAPK expressions in HG-induced human umbilical vein endothelial cells (HUVECs).The results indicated that icariin significantly ameliorated the weight loss and dampened the increase in water intake of the diabetic mice.Meanwhile, icariin had a certain ameliorative effect on blood glucose and food intake without significant difference.The results of isolated thoracic aortas vascular rings contraction and vasodilation function indicated that icariin significantly improved Phe-induced vascular contraction and Ach‐induced vascular relaxation.Meanwhile, icariin had a certain ameliorative effect on KCl-induced vascular contraction response without significant difference.However, no significant change was observed on endothelium‐independent vascular rings relaxation response induced by SNP after treatment with icariin.Results of Western blot showed that icariin inhibited the expression of p-p38 MAPK and induced expression of p-eNOS in the high glucose-induced HUVECs cell model.Therefore, icariin may attenuate alloxan-induced type 1 diabetic mice vascular diastolic function by inhibiting expression of p-p38 MAPK and inducing expression of p-eNOS.
- icariin,
- alloxan,
- diabetic mouse model,
- vascular endothelial function

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[1]	. BMJ，2020，369：m997.
[2]	Tseng CH. Mortality and causes of death in a national sample of diabetic patients in Taiwan［J］. Diabetes Care，2004，27（7）：1605-1609.
[3]	Song LJ，Chen X，Mi L，et al. Icariin-induced inhibition of SIRT6/NF-κB triggers redox mediated apoptosis and enhances anti-tumor immunity in triple-negative breast cancer［J］. Cancer Sci，2020，111（11）：4242-4256.
[4]	Qi SS，He J，Zheng HX，et al. Icariin prevents diabetes-induced bone loss in rats by reducing blood glucose and suppressing bone turnover［J］. Molecules，2019，24（10）：1871.
[5]	Cao LH，Qiao JY，Huang HY，et al. PI3K-AKT signaling activation and icariin：the potential effects on the perimenopausal depression-like rat model［J］. Molecules，2019，24（20）：3700.
[6]	Angeloni C，Barbalace MC，Hrelia S. Icariin and its metabolites as potential protective phytochemicals against Alzheimer''s disease［J］. Front Pharmacol，2019，10：271.
[7]	Li X，Wang YX，Shi P，et al. Icariin treatment reduces blood glucose levels in type 2 diabetic rats and protects pancreatic function［J］. Exp Ther Med，2020，19（4）：2690-2696.
[8]	Qiao C，Wang H，Song ZY，et al. Icariin attenuates diabetic cardiomyopathy and downregulates extracellular matrix proteins in heart tissue of type 2 diabetic rats［J］. Pharmacology，2020，105（9/10）：576-585.
[9]	Yao WH，Wang K，Wang XN，et al. Icariin ameliorates endothelial dysfunction in type 1 diabetic rats by suppressing ER stress via the PPARα/Sirt1/AMPKα pathway［J］. J Cell Physiol，2021，236（3）：1889-1902.
[10]	Chen SS，Wang ZY，Zhou H，et al. Icariin reduces high glucose-induced endothelial progenitor cell dysfunction via inhibiting the p38/CREB pathway and activating the Akt/eNOS/NO pathway［J］. Exp Ther Med，2019，18（6）：4774-4780.
[11]	Huang ZJ，Ji H，Li P，et al. Hypoglycemic effect and mechanism of polysaccharides from cultured mycelium of cordyceps sinensis［J］. J China Pharm Univ（中国药科大学学报），2002，33（1）：51-54.
[12]	Jia GH，Hill MA，Sowers JR. Diabetic cardiomyopathy：an update of mechanisms contributing to this clinical entity［J］. Circ Res，2018，122（4）：624-638.
[13]	Shi Y，Vanhoutte PM. Macro- and microvascular endothelial dysfunction in diabetes［J］. J Diabetes，2017，9（5）：434-449.
[14]	Vita JA，Keaney JFJr. Endothelial function：a barometer for cardiovascular risk［J］？ Circulation，2002，106（6）：640-642.
[15]	Xiao HB，Liu ZK，Lu XY，et al. Icariin regulates PRMT/ADMA/DDAH pathway to improve endothelial function［J］. Pharmacol Rep，2015，67（6）：1147-1154.
[16]	Vanhoutte PM. Endothelial control of vasomotor function：from health to coronary disease［J］. Circ J，2003，67（7）：572-575.
[17]	Lee YN，Im E. Regulation of miRNAs by natural antioxidants in cardiovascular diseases：focus on SIRT1 and eNOS［J］. Antioxidants （Basel），2021，10（3）：377.
[18]	Papaconstantinou J. The role of signaling pathways of inflammation and oxidative stress in development of senescence and aging phenotypes in cardiovascular disease［J］. Cells，2019，8（11）：1383.
[19]	Leng B，Li C，Sun Y，et al. Protective effect of astragaloside IV on high glucose-induced endothelial dysfunction via inhibition of P2X7R dependent P38 MAPK signaling pathway［J］. Oxid Med Cell Longev，2020，2020：5070415.
[20]	Steven S，Frenis K，Oelze M，et al. Vascular inflammation and oxidative stress：major triggers for cardiovascular disease［J］. Oxid Med Cell Longev，2019，2019：7092151.

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