Metabolomic study on clinical staging of methamphetamine detoxification by LC-QTOF-MS

WANG Jingqi; WANG Ye; ZHANG Yi; LIANG Juncheng; DENG Yanping; HANG Taijun; SONG Min

doi:10.11665/j.issn.1000-5048.20220309

Journal of China Pharmaceutical University > 2022 > 53(3): 314-322. > DOI: 10.11665/j.issn.1000-5048.20220309

WANG Jingqi, WANG Ye, ZHANG Yi, LIANG Juncheng, DENG Yanping, HANG Taijun, SONG Min. Metabolomic study on clinical staging of methamphetamine detoxification by LC-QTOF-MS[J]. Journal of China Pharmaceutical University, 2022, 53(3): 314-322. DOI: 10.11665/j.issn.1000-5048.20220309

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Metabolomic study on clinical staging of methamphetamine detoxification by LC-QTOF-MS

1.
School of Pharmacy, China Pharmaceutical University, Nanjing 210009
2.
Office of China National Narcotics Control Commission-China Pharmaceutical University Joint Laboratory on Key Technologies of Narcotics Control, Beijing 100193
3.
National Institute on Drug Dependence, Peking University,Beijing 100191, China

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Received Date: November 10, 2021
Revised Date: April 15, 2022

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Abstract

Abstract

At present , methamphetamine has become a major hidden danger in global public health safety. In order to judge methamphetamine addicts and methamphetamine abstainers more scientifically and reliably, this study analyzed the endogenous metabolites in plasma, serum and urine of methamphetamine addicts, methamphetamine abstainers and healthy volunteers by highly sensitive high-throughput liquid chromatography quadrupole time-of-flight mass spectrometry (LC-QTOF-MS) analytical instrument. The obtained metabolomic data were processed by univariate analysis (t-test) and multivariate analysis (PLS-DA and OPLS-DA) and eligible potential biomarkers were then screened.The identified biomarkers set enrichment analysis to find the connection between metabolites and metabolic pathways.Multivariate statistical results showed that methamphetamine acute group, recovery group and healthy group were clearly separated.3, 18 and 6 regulated metabolites were identified in serum, plasma and urine, respectively, suggesting that lipid metabolism was abnormal in methamphetamine acute group, and that fatty acid metabolism, sulfate/sulfite metabolism and sex hormone metabolism were abnormal in methamphetamine recovery group.The selected potential biomarkers in this study provide the possibility for scientific judgment of the clinical stage of methamphetamine detoxification.
- methamphetamine,
- LC-QTOF-MS,
- multivariate analysis,
- biomarker,
- clinical stage of detoxification

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References

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