Effects and mechanisms of SNP-9 on Aβ<sub>25-35</sub>-induced damage in bEnd.3 cells

MENG Yue; YAO Siyuan; GAO Xiangdong; CHEN Song

doi:10.11665/j.issn.1000-5048.20220311

Journal of China Pharmaceutical University > 2022 > 53(3): 333-339. > DOI: 10.11665/j.issn.1000-5048.20220311

MENG Yue, YAO Siyuan, GAO Xiangdong, CHEN Song. Effects and mechanisms of SNP-9 on Aβ_25-35-induced damage in bEnd.3 cells[J]. Journal of China Pharmaceutical University, 2022, 53(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20220311

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Effects and mechanisms of SNP-9 on Aβ_25-35-induced damage in bEnd.3 cells

Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Natural Science Foundation of China (No.82073755, No.82173728, No.81872850)

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Received Date: January 18, 2022
Revised Date: May 10, 2022

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Abstract

Abstract

In order to investigate the effects of neuroprotective peptide SNP-9 which is derived from silk fibroin hydrolysate on the injury of the blood-brain barrier in Alzheimer′s disease (AD), Aβ_25-35 was used to damage brain microvascular endothelial cells bEnd.3 to establish AD injury model and drug intervention was performed.MTT assay was used to detect the effects of SNP-9 and Aβ_25-35 on cell viability.RT-qPCR was used to determine the effects of SNP-9 and Aβ_25-35on the mRNA levels of tight junctions (TJs)-related ZO-1, occludin and claudin-5.Western blot was used to detect the effects of SNP-9 and Aβ_25-35 on the protein levels of TNF-α, phosphorylated NF-κB, NF-κB, IκBα and RAGE.The results showed that SNP-9 reduced bEnd.3 cell damage induced by Aβ_25-35, and improved the abnormal mRNA levels of ZO-1, occludin and claudin-5 in model cells.It alleviated the abnormal protein levels of TNF-α, phosphorylated NF-κB, IκBα and RAGE induced by Aβ_25-35. These results suggest that SNP-9 may regulate the levels of TNF-α in model cells by influencing RAGE/NF-κB pathway, and then ameliorate TJs-related abnormalities and alleviate bEnd.3 cell injury induced by Aβ_25-35.
- SNP-9,
- Alzheimer′s disease,
- blood-brain barrier,
- bEnd.3,
- Aβ_25-35,
- NF-κB

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Effects and mechanisms of SNP-9 on Aβ25-35-induced damage in bEnd.3 cells

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Effects and mechanisms of SNP-9 on Aβ_25-35-induced damage in bEnd.3 cells