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MENG Yue, YAO Siyuan, GAO Xiangdong, CHEN Song. Effects and mechanisms of SNP-9 on Aβ25-35-induced damage in bEnd.3 cells[J]. Journal of China Pharmaceutical University, 2022, 53(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20220311
Citation: MENG Yue, YAO Siyuan, GAO Xiangdong, CHEN Song. Effects and mechanisms of SNP-9 on Aβ25-35-induced damage in bEnd.3 cells[J]. Journal of China Pharmaceutical University, 2022, 53(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20220311

Effects and mechanisms of SNP-9 on Aβ25-35-induced damage in bEnd.3 cells

Funds: This study was supported by the National Natural Science Foundation of China (No.82073755, No.82173728, No.81872850)
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  • Received Date: January 18, 2022
  • Revised Date: May 10, 2022
  • In order to investigate the effects of neuroprotective peptide SNP-9 which is derived from silk fibroin hydrolysate on the injury of the blood-brain barrier in Alzheimer′s disease (AD), Aβ25-35 was used to damage brain microvascular endothelial cells bEnd.3 to establish AD injury model and drug intervention was performed.MTT assay was used to detect the effects of SNP-9 and Aβ25-35 on cell viability.RT-qPCR was used to determine the effects of SNP-9 and Aβ25-35 on the mRNA levels of tight junctions (TJs)-related ZO-1, occludin and claudin-5.Western blot was used to detect the effects of SNP-9 and Aβ25-35 on the protein levels of TNF-α, phosphorylated NF-κB, NF-κB, IκBα and RAGE.The results showed that SNP-9 reduced bEnd.3 cell damage induced by Aβ25-35, and improved the abnormal mRNA levels of ZO-1, occludin and claudin-5 in model cells.It alleviated the abnormal protein levels of TNF-α, phosphorylated NF-κB, IκBα and RAGE induced by Aβ25-35. These results suggest that SNP-9 may regulate the levels of TNF-α in model cells by influencing RAGE/NF-κB pathway, and then ameliorate TJs-related abnormalities and alleviate bEnd.3 cell injury induced by Aβ25-35.
  • [1]
    . Alzheimers Dement,2021,17(3):327-406.
    [2]
    Jeong S. Molecular and cellular basis of neurodegeneration in Alzheimer′s disease[J]. Mol Cells,2017,40(9):613-620.
    [3]
    Sweeney MD,Montagne A,Sagare AP,et al. Vascular dysfunction — The disregarded partner of Alzheimer′s disease[J]. Alzheimers Dement,2019,15(1):158-167.
    [4]
    Elahi FM,Casaletto KB,la Joie R,et al. Plasma biomarkers of astrocytic and neuronal dysfunction in early- and late-onset Alzheimer′s disease[J]. Alzheimers Dement,2020,16(4):681-695.
    [5]
    Nation DA,Sweeney MD,Montagne A,et al. Blood-brain barrier breakdown is an early biomarker of human cognitive dysfunction[J]. Nat Med,2019,25(2):270-276.
    [6]
    Serlin Y,Shelef I,Knyazer B,et al. Anatomy and physiology of the blood-brain barrier[J]. Semin Cell Dev Biol,2015,38:2-6.
    [7]
    Cai ZY,Qiao PF,Wan CQ,et al. Role of blood-brain barrier in Alzheimer′s disease[J]. J Alzheimers Dis,2018,63(4):1223-1234.
    [8]
    Sweeney MD,Sagare AP,Zlokovic BV. Blood-brain barrier breakdown in Alzheimer disease and other neurodegenerative disorders[J]. Nat Rev Neurol,2018,14(3):133-150.
    [9]
    Iadecola C. The neurovascular unit coming of age:a journey through neurovascular coupling in health and disease[J]. Neuron,2017,96(1):17-42.
    [10]
    Zenaro E,Piacentino G,Constantin G. The blood-brain barrier in Alzheimer′s disease[J]. Neurobiol Dis,2017,107:41-56.
    [11]
    Erd? F,Denes L,de Lange E. Age-associated physiological and pathological changes at the blood-brain barrier:a review[J]. J Cereb Blood Flow Metab,2017,37(1):4-24.
    [12]
    Song J,Choi SM,Whitcomb DJ,et al. Adiponectin controls the apoptosis and the expression of tight junction proteins in brain endothelial cells through AdipoR1 under beta amyloid toxicity[J]. Cell Death Dis,2017,8(10):e3102.
    [13]
    Mangialasche F,Solomon A,Winblad B,et al. Alzheimer′s disease:clinical trials and drug development[J]. Lancet Neurol,2010,9(7):702-716.
    [14]
    Breijyeh Z,Karaman R. Comprehensive review on Alzheimer′s disease:causes and treatment[J]. Molecules,2020,25(24):5789.
    [15]
    Xu Z,Chen S,Wang Y,et al. Neuroprotective effects of silk fibroin hydrolysate against Aβ25-35 induced cytotoxicity in SH-SY5Y cells and primary hippocampal neurons by regulating ROS inactivation of PP2A[J]. J Funct Foods,2018,45:100-109.
    [16]
    Yao SY,Xu Z,Chen S,et al. Silk fibroin hydrolysate improves memory impairment via multi-target function[J]. J Funct Foods,2022,89:104942.
    [17]
    Greene C,Hanley N,Campbell M. Claudin-5:gatekeeper of neurological function[J]. Fluids Barriers CNS,2019,16(1):3.
    [18]
    Yamazaki Y,Shinohara M,Shinohara M,et al. Selective loss of cortical endothelial tight junction proteins during Alzheimer′s disease progression[J]. Brain,2019,142(4):1077-1092.
    [19]
    Huang XW,Hussain B,Chang JL. Peripheral inflammation and blood-brain barrier disruption:effects and mechanisms[J]. CNS Neurosci Ther,2021,27(1):36-47.
    [20]
    Voirin AC,Perek N,Roche F. Inflammatory stress induced by a combination of cytokines (IL-6,IL-17,TNF-α) leads to a loss of integrity on bEnd.3 endothelial cells in vitro BBB model[J]. Brain Res,2020,1730:146647.
    [21]
    Feng S,Zou L,Wang HJ,et al. RhoA/ROCK-2 pathway inhibition and tight junction protein upregulation by catalpol suppresses lipopolysaccaride-induced disruption of blood-brain barrier permeability[J]. Molecules,2018,23(9):2371.
    [22]
    Wang L,Zhang R,Chen J,et al. Baicalin protects against TNF-α-induced injury by down-regulating miR-191a that targets the tight junction protein ZO-1 in IEC-6 cells[J]. Biol Pharm Bull,2017,40(4):435-443.
    [23]
    Burek M,F?rster CY. Cloning and characterization of the murine claudin-5 promoter[J]. Mol Cell Endocrinol,2009,298(1/2):19-24.
    [24]
    Hayden MS,Ghosh S. Regulation of NF-κB by TNF family cytokines[J]. Semin Immunol,2014,26(3):253-266.
    [25]
    Caldwell AB,Cheng Z,Vargas JD,et al. Network dynamics determine the autocrine and paracrine signaling functions of TNF[J]. Genes Dev,2014,28(19):2120-2133.
    [26]
    Zhang Q,Lenardo MJ,Baltimore D. 30 years of NF-κB:a blossoming of relevance to human pathobiology[J]. Cell,2017,168(1/2):37-57.
    [27]
    McNamara AJ,Danthi P. Loss of IKK subunits limits NF-κB signaling in reovirus-infected cells[J]. J Virol,2020,94(10):e00382-e00320.
    [28]
    Chen YJ,Chan DC,Chiang CK,et al. Advanced glycation end-products induced VEGF production and inflammatory responses in human synoviocytes via RAGE-NF-κB pathway activation[J]. J Orthop Res,2016,34(5):791-800.
    [29]
    Ding B,Lin CH,Liu Q,et al. Tanshinone IIA attenuates neuroinflammation via inhibiting RAGE/NF-κB signaling pathway in vivo and in vitro[J]. J Neuroinflammation,2020,17(1):302.
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