• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
MENG Yue, YAO Siyuan, GAO Xiangdong, CHEN Song. Effects and mechanisms of SNP-9 on Aβ25-35-induced damage in bEnd.3 cells[J]. Journal of China Pharmaceutical University, 2022, 53(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20220311
Citation: MENG Yue, YAO Siyuan, GAO Xiangdong, CHEN Song. Effects and mechanisms of SNP-9 on Aβ25-35-induced damage in bEnd.3 cells[J]. Journal of China Pharmaceutical University, 2022, 53(3): 333-339. DOI: 10.11665/j.issn.1000-5048.20220311
shu

Effects and mechanisms of SNP-9 on Aβ25-35-induced damage in bEnd.3 cells

  • Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198, China

Funds: This study was supported by the National Natural Science Foundation of China (No.82073755, No.82173728, No.81872850)
More Information
  • Received Date: January 18, 2022
  • Revised Date: May 10, 2022
    Graphical Abstract
    • Abstract
  • In order to investigate the effects of neuroprotective peptide SNP-9 which is derived from silk fibroin hydrolysate on the injury of the blood-brain barrier in Alzheimer′s disease (AD), Aβ25-35 was used to damage brain microvascular endothelial cells bEnd.3 to establish AD injury model and drug intervention was performed.MTT assay was used to detect the effects of SNP-9 and Aβ25-35 on cell viability.RT-qPCR was used to determine the effects of SNP-9 and Aβ25-35 on the mRNA levels of tight junctions (TJs)-related ZO-1, occludin and claudin-5.Western blot was used to detect the effects of SNP-9 and Aβ25-35 on the protein levels of TNF-α, phosphorylated NF-κB, NF-κB, IκBα and RAGE.The results showed that SNP-9 reduced bEnd.3 cell damage induced by Aβ25-35, and improved the abnormal mRNA levels of ZO-1, occludin and claudin-5 in model cells.It alleviated the abnormal protein levels of TNF-α, phosphorylated NF-κB, IκBα and RAGE induced by Aβ25-35. These results suggest that SNP-9 may regulate the levels of TNF-α in model cells by influencing RAGE/NF-κB pathway, and then ameliorate TJs-related abnormalities and alleviate bEnd.3 cell injury induced by Aβ25-35.
    • FullText(HTML)
    • References (29)
  • [1]
    . Alzheimers Dement，2021，17（3）：327-406.
    [2]
    Jeong S. Molecular and cellular basis of neurodegeneration in Alzheimer′s disease［J］. Mol Cells，2017，40（9）：613-620.
    [3]
    Sweeney MD，Montagne A，Sagare AP，et al. Vascular dysfunction — The disregarded partner of Alzheimer′s disease［J］. Alzheimers Dement，2019，15（1）：158-167.
    [4]
    Elahi FM，Casaletto KB，la Joie R，et al. Plasma biomarkers of astrocytic and neuronal dysfunction in early- and late-onset Alzheimer′s disease［J］. Alzheimers Dement，2020，16（4）：681-695.
    [5]
    Nation DA，Sweeney MD，Montagne A，et al. Blood-brain barrier breakdown is an early biomarker of human cognitive dysfunction［J］. Nat Med，2019，25（2）：270-276.
    [6]
    Serlin Y，Shelef I，Knyazer B，et al. Anatomy and physiology of the blood-brain barrier［J］. Semin Cell Dev Biol，2015，38：2-6.
    [7]
    Cai ZY，Qiao PF，Wan CQ，et al. Role of blood-brain barrier in Alzheimer′s disease［J］. J Alzheimers Dis，2018，63（4）：1223-1234.
    [8]
    Sweeney MD，Sagare AP，Zlokovic BV. Blood-brain barrier breakdown in Alzheimer disease and other neurodegenerative disorders［J］. Nat Rev Neurol，2018，14（3）：133-150.
    [9]
    Iadecola C. The neurovascular unit coming of age：a journey through neurovascular coupling in health and disease［J］. Neuron，2017，96（1）：17-42.
    [10]
    Zenaro E，Piacentino G，Constantin G. The blood-brain barrier in Alzheimer′s disease［J］. Neurobiol Dis，2017，107：41-56.
    [11]
    Erd? F，Denes L，de Lange E. Age-associated physiological and pathological changes at the blood-brain barrier：a review［J］. J Cereb Blood Flow Metab，2017，37（1）：4-24.
    [12]
    Song J，Choi SM，Whitcomb DJ，et al. Adiponectin controls the apoptosis and the expression of tight junction proteins in brain endothelial cells through AdipoR1 under beta amyloid toxicity［J］. Cell Death Dis，2017，8（10）：e3102.
    [13]
    Mangialasche F，Solomon A，Winblad B，et al. Alzheimer′s disease：clinical trials and drug development［J］. Lancet Neurol，2010，9（7）：702-716.
    [14]
    Breijyeh Z，Karaman R. Comprehensive review on Alzheimer′s disease：causes and treatment［J］. Molecules，2020，25（24）：5789.
    [15]
    Xu Z，Chen S，Wang Y，et al. Neuroprotective effects of silk fibroin hydrolysate against Aβ25-35 induced cytotoxicity in SH-SY5Y cells and primary hippocampal neurons by regulating ROS inactivation of PP2A［J］. J Funct Foods，2018，45：100-109.
    [16]
    Yao SY，Xu Z，Chen S，et al. Silk fibroin hydrolysate improves memory impairment via multi-target function［J］. J Funct Foods，2022，89：104942.
    [17]
    Greene C，Hanley N，Campbell M. Claudin-5：gatekeeper of neurological function［J］. Fluids Barriers CNS，2019，16（1）：3.
    [18]
    Yamazaki Y，Shinohara M，Shinohara M，et al. Selective loss of cortical endothelial tight junction proteins during Alzheimer′s disease progression［J］. Brain，2019，142（4）：1077-1092.
    [19]
    Huang XW，Hussain B，Chang JL. Peripheral inflammation and blood-brain barrier disruption：effects and mechanisms［J］. CNS Neurosci Ther，2021，27（1）：36-47.
    [20]
    Voirin AC，Perek N，Roche F. Inflammatory stress induced by a combination of cytokines （IL-6，IL-17，TNF-α） leads to a loss of integrity on bEnd.3 endothelial cells in vitro BBB model［J］. Brain Res，2020，1730：146647.
    [21]
    Feng S，Zou L，Wang HJ，et al. RhoA/ROCK-2 pathway inhibition and tight junction protein upregulation by catalpol suppresses lipopolysaccaride-induced disruption of blood-brain barrier permeability［J］. Molecules，2018，23（9）：2371.
    [22]
    Wang L，Zhang R，Chen J，et al. Baicalin protects against TNF-α-induced injury by down-regulating miR-191a that targets the tight junction protein ZO-1 in IEC-6 cells［J］. Biol Pharm Bull，2017，40（4）：435-443.
    [23]
    Burek M，F?rster CY. Cloning and characterization of the murine claudin-5 promoter［J］. Mol Cell Endocrinol，2009，298（1/2）：19-24.
    [24]
    Hayden MS，Ghosh S. Regulation of NF-κB by TNF family cytokines［J］. Semin Immunol，2014，26（3）：253-266.
    [25]
    Caldwell AB，Cheng Z，Vargas JD，et al. Network dynamics determine the autocrine and paracrine signaling functions of TNF［J］. Genes Dev，2014，28（19）：2120-2133.
    [26]
    Zhang Q，Lenardo MJ，Baltimore D. 30 years of NF-κB：a blossoming of relevance to human pathobiology［J］. Cell，2017，168（1/2）：37-57.
    [27]
    McNamara AJ，Danthi P. Loss of IKK subunits limits NF-κB signaling in reovirus-infected cells［J］. J Virol，2020，94（10）：e00382-e00320.
    [28]
    Chen YJ，Chan DC，Chiang CK，et al. Advanced glycation end-products induced VEGF production and inflammatory responses in human synoviocytes via RAGE-NF-κB pathway activation［J］. J Orthop Res，2016，34（5）：791-800.
    [29]
    Ding B，Lin CH，Liu Q，et al. Tanshinone IIA attenuates neuroinflammation via inhibiting RAGE/NF-κB signaling pathway in vivo and in vitro［J］. J Neuroinflammation，2020，17（1）：302.
    • Related Articles

  • Related Articles

    [1]PAN Kemeng, CHEN Song, GAO Xiangdong, YAO Wenbing. Effects and mechanisms of microRNA-23b on neuronal apoptosis induced by Aβ25-35[J]. Journal of China Pharmaceutical University, 2021, 52(4): 480-486. DOI: 10.11665/j.issn.1000-5048.20210411
    [2]SUN Yan, GAO Xiangdong, CHEN Song. Effect and mechanism of FGF21 on astrocyte damage induced by Aβ25-35[J]. Journal of China Pharmaceutical University, 2019, 50(4): 490-496. DOI: 10.11665/j.issn.1000-5048.20190415
    [3]PENG Xuehan, XIE Wenmin, LI Ji, YU Feng. Comparative investigation of in vitro antitumor activity of Ganoderma lucidum spore oil[J]. Journal of China Pharmaceutical University, 2019, 50(1): 81-86. DOI: 10.11665/j.issn.1000-5048.20190111
    [4]YU Haiyang, CHEN Song, XU Zheng, GAO Xiangdong, YAO Wenbing. Protective effect of fibroin peptides on Aβ25-35-induced injury in SH-SY5Y cells and its mechanism[J]. Journal of China Pharmaceutical University, 2017, 48(5): 609-613. DOI: 10.11665/j.issn.1000-5048.20170517
    [5]WANG Xiao-jie, XINHUA Nabi, SUN Yu. Effects of active peptide extracted from cheese whey on the expression of PPARγ mRNA and NF-κB activity in 3T3-L1 adipocytes[J]. Journal of China Pharmaceutical University, 2012, 43(5): 449-452.
    [6]CHEN Hong, YANG Jie, CUI Wei-xi, WANG Qiang. Effects and mechanism of ursolic acid on lipopolysaccharide-induced THP-1 cells[J]. Journal of China Pharmaceutical University, 2011, 42(5): 447-451.
    [7]XU Ling-jie, LI Cong, ZHANG Mian, DAI Yue, HUANG Fang. Effect of the ethyl acetate extract of Farfarae Flos on inflammatory factors[J]. Journal of China Pharmaceutical University, 2011, 42(1): 64-67.
    [8]Transcription factors as new targets for cancer therapy[J]. Journal of China Pharmaceutical University, 2010, 41(2): 97-103.
    [10]Effect of Nerve Regeneration Factor on GAP-43 and NF-L Gene Expression in Dorsal Roots Ganglia Cell[J]. Journal of China Pharmaceutical University, 2001, (3): 73-76.

  • Cited by

    Periodical cited type(3)

    1. 何燕琳，黄朝瑜，杨香，王晨，邵方娴，严菲. UPLC-MS/MS检测达格列净中的基因毒性杂质5-溴-2-氯苯甲酰氯. 药物生物技术. 2025(02): 168-172 .
    2. 沈丹丹，胡振晶，曾令高，唐华. 国产艾司唑仑原料药及其片剂关键质量属性研究进展. 中国药业. 2024(03): 19-25 .
    3. 邵方娴，何燕琳，芦丽，黄朝瑜，王晨，严菲. 柱前衍生化液质联用法测定氯乙酰氯试剂中的乙酰氯和二氯乙酰氯. 化学试剂. 2024(04): 102-107 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views (149) PDF downloads (336) Cited by(3)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：365451

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return