• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
LIU Yanjiao, WEN Cheng, LI Dan, GAO Pei, ZHU Guodong. Secretory phospholipase A2 responsiveness and in vitro anti-tumor activity of oxaliplatin-loaded liposomes modified with facial amphiphiles[J]. Journal of China Pharmaceutical University, 2022, 53(4): 441-451. DOI: 10.11665/j.issn.1000-5048.20220407
Citation: LIU Yanjiao, WEN Cheng, LI Dan, GAO Pei, ZHU Guodong. Secretory phospholipase A2 responsiveness and in vitro anti-tumor activity of oxaliplatin-loaded liposomes modified with facial amphiphiles[J]. Journal of China Pharmaceutical University, 2022, 53(4): 441-451. DOI: 10.11665/j.issn.1000-5048.20220407
shu

Secretory phospholipase A2 responsiveness and in vitro anti-tumor activity of oxaliplatin-loaded liposomes modified with facial amphiphiles

  • School of Biotechnology and Health Sciences, Wuyi University, Jiangmen 529030, China

Funds: This study was supported by the National Natural Science Foundation of China (No.8167131086)
More Information
  • Received Date: February 24, 2022
  • Revised Date: June 01, 2022
    Graphical Abstract
    • Abstract
  • Modulating drug release from liposomes at tumor sites are important for eliciting therapeutic effects of platinum drugs considering their low permeability through liposomal membranes, here a novel secretory phospholipase A2 (sPLA2) responsive-liposome system was constructed for oxaliplatin (L-OHP).Lipid ingredients dipalmitoyl phosphatidylcholine and distearoyl phosphoethanolamine-PEG2k, together with facial amphiphiles (FAs) including lithocholic acid (LCA) or 3-keto lithocholic acid (kLCA) were used to prepare sPLA2 responsive-liposome (LCA-Lip or kLCA-Lip) by thin-film hydration method.The physicochemical properties, sPLA2-responsive drug release and anti-tumor activity were evaluated in vitro.The results indicated L-OHP loaded liposomes modified with FAs had similar particle sizes of approximately 100 nm and narrow size distributions (PDI < 0.11).Compared with non-FAs-containing liposomes (C-Lip), LCA-Lip or kLCA-Lip has a comparable entrapment efficiency and loading efficiency.LCA-Lip or kLCA-Lip didn't show significant higher drug leakage at the presence of 10% or 50% fetal bovine serum (FBS) in media than that in media without FBS.Treated with secretory phospholipase A2 from Colo205 cells culture conditioned medium (CCM sPLA2) for 24 h, FAs modified liposomes released about 70% of carboxyfluorescein (CF), while C-Lip only released 20% of CF.Compared to L-OHP loaded C-Lip, L-OHP-loaded FAs-included formulations had much greater anti-proliferative activity against sPLA2-secreting Colo205 cells.In summary, our results shows that LCA or kLCA promotes responsiveness of liposomes to tumor-related sPLA2 and points to a new way to develop platium drugs-loaded liposomal delivery systems with better release mechanisms.
    • FullText(HTML)
    • References (36)
  • [1]
    . Russ J Bioorg Chem，2018，44（6）：619-630.
    [2]
    Li YN，Xu PC，He DS，et al. Long-circulating thermosensitive liposomes for the targeted drug delivery of oxaliplatin［J］. Int J Nanomedicine，2020，15：6721-6734.
    [3]
    Seetharamu N，Kim E，Hochster H，et al. Phase II study of liposomal cisplatin （SPI-77） in platinum-sensitive recurrences of ovarian cancer［J］. Anticancer Res，2010，30（2）：541-545.
    [4]
    Yamashita S，Yamashita J，Sakamoto K，et al. Increased expression of membrane-associated phospholipase A2 shows malignant potential of human breast cancer cells［J］. Cancer，1993，71（10）：3058-3064.
    [5]
    Jiang JZ，Neubauer BL，Graff JR，et al. Expression of group IIA secretory phospholipase A2 is elevated in prostatic intraepithelial neoplasia and adenocarcinoma［J］. Am J Pathol，2002，160（2）：667-671.
    [6]
    Kiyohara H，Egami H，Kako H，et al. Immunohistochemical localization of group II phospholipase A2 in human pancreatic carcinomas［J］. Int J Pancreatol，1993，13（1）：49-57.
    [7]
    Abe T，Sakamoto K，Kamohara H，et al. Group II phospholipase A2 is increased in peritoneal and pleural effusions in patients with various cancers［J］. Int Congr Ser，2003，1255：351-360.
    [8]
    Davidsen J，J?rgensen K，Andresen TL，et al. Secreted phospholipase A2 as a new enzymatic trigger mechanism for localised liposomal drug release and absorption in diseased tissue［J］. Biochim Biophys Acta BBA Biomembr，2003，1609（1）：95-101.
    [9]
    Zhu GD，Mock JN，Aljuffali I，et al. Secretory phospholipase A2 responsive liposomes［J］. J Pharm Sci，2011，100（8）：3146-3159.
    [10]
    Pourhassan H，Clergeaud G，Hansen AE，et al. Revisiting the use of sPLA2-sensitive liposomes in cancer therapy［J］. J Control Release，2017，261：163-173.
    [11]
    Andresen TL，Davidsen J，Begtrup M，et al. Enzymatic release of antitumor ether lipids by specific phospholipase A2 activation of liposome-forming prodrugs［J］. J Med Chem，2004，47（7）：1694-1703.
    [12]
    De Jonge MJA，Slingerland M，Loos WJ，et al. Early cessation of the clinical development of LiPlaCis，a liposomal cisplatin formulation［J］. Eur J Cancer，2010，46（16）：3016-3021.
    [13]
    Moghimipour E，Ameri A，Handali S. Absorption-enhancing effects of bile salts［J］. Molecules，2015，20（8）：14451-14473.
    [14]
    De Luca D，Minucci A，Zecca E，et al. Bile acids cause secretory phospholipase A2 activity enhancement，revertible by exogenous surfactant administration［J］. Intensive Care Med，2009，35（2）：321-326.
    [15]
    Cui JG，Fan L，Huang LL，et al. Synthesis and evaluation of some steroidal oximes as cytotoxic agents：structure/activity studies （I）［J］. Steroids，2009，74（1）：62-72.
    [16]
    Bartlett GR. Phosphorus assay in column chromatography［J］. J Biol Chem，1959，234（3）：466-468.
    [17]
    Li JY，Han JY. Determination of oxalipltin and relative impurity by HPLC［J］. Her Med（医药导报），2008，27（5）：597-598.
    [18]
    Sait? H，Sugimoto Y，Tabeta R，et al. Incorporation of bile acid of low concentration into model and biological membranes studied by 2H and 31P NMR［J］. J Biochem，1983，94（6）：1877-1887.
    [19]
    Klibanov AL，Maruyama K，Torchilin VP，et al. Amphipathic polyethyleneglycols effectively prolong the circulation time of liposomes［J］. FEBS Lett，1990，268（1）：235-237.
    [20]
    Hatakeyama H，Akita H，Ito E，et al. Systemic delivery of siRNA to tumors using a lipid nanoparticle containing a tumor-specific cleavable PEG-lipid［J］. Biomaterials，2011，32（18）：4306-4316.
    [21]
    Trilli J，Caramazza L，Paolicelli P，et al. The impact of bilayer rigidity on the release from magnetoliposomes vesicles controlled by PEMFs［J］. Pharmaceutics，2021，13（10）：1712.
    [22]
    Murakami M，Sato H，Miki Y，et al. A new era of secreted phospholipase A?［J］. J Lipid Res，2015，56（7）：1248-1261.
    [23]
    Murase R，Taketomi Y，Miki Y，et al. Group III phospholipase A2 promotes colitis and colorectal cancer［J］. Sci Rep，2017，7（1）：12261.
    [24]
    Wilschut JC，Regts J，Scherphof G. Action of phospholipase A2 on phospholipid vesicles. Preservation of the membrane permeability barrier during asymmetric bilayer degradation［J］. FEBS Lett，1979，98（1）：181-186.
    [25]
    Boyanovsky BB，Webb NR. Biology of secretory phospholipase A2［J］. Cardiovasc Drugs Ther，2009，23（1）：61-72.
    [26]
    Jensen SS，Andresen TL，Davidsen J，et al. Secretory phospholipase A2 as a tumor-specific trigger for targeted delivery of a novel class of liposomal prodrug anticancer etherlipids［J］. Mol Cancer Ther，2004，3（11）：1451-1458.
    [27]
    Murakami M，Masuda S，Shimbara S，et al. Cellular distribution，post-translational modification，and tumorigenic potential of human group III secreted phospholipase A2［J］. J Biol Chem，2005，280（26）：24987-24998.
    [28]
    Ramana LN，Sharma S，Sethuraman S，et al. Investigation on the stability of saquinavir loaded liposomes：implication on stealth，release characteristics and cytotoxicity［J］. Int J Pharm，2012，431（1/2）：120-129.
    [29]
    Li SH，Goins B，Phillips WT，et al. Remote-loading labeling of liposomes with 99mTc-BMEDA and its stability evaluation：effects of lipid formulation and pH/chemical gradient［J］. J Liposome Res，2011，21（1）：17-27.
    [30]
    Matloub AA，Salama AH，Aglan HA，et al. Exploiting bilosomes for delivering bioactive polysaccharide isolated from Enteromorpha intestinalis for hacking hepatocellular carcinoma［J］. Drug Dev Ind Pharm，2018，44（4）：523-534.
    [31]
    Zafar A，Alruwaili NK，Imam SS，et al. Development and evaluation of luteolin loaded pegylated bilosome：optimization，in vitro characterization，and cytotoxicity study［J］. Drug Deliv，2021，28（1）：2562-2573.
    [32]
    Abbas H，El-Feky YA，Al-Sawahli MM，et al. Development and optimization of curcumin analog nano-bilosomes using 2 1.3 1 full factorial design for anti-tumor profiles improvement in human hepatocellular carcinoma：in-vitro evaluation，in-vivo safety assay［J］. Drug Deliv，2022，29（1）：714-727.
    [33]
    Jiang L，Li L，He XD，et al. Overcoming drug-resistant lung cancer by paclitaxel loaded dual-functional liposomes with mitochondria targeting and pH-response［J］. Biomaterials，2015，52：126-139.
    [34]
    Herr I，Debatin KM. Cellular stress response and apoptosis in cancer therapy［J］. Blood，2001，98（9）：2603-2614.
    [35]
    ?strem RG，Parhamifar L，Pourhassan H，et al. Secretory phospholipase A2 responsive liposomes exhibit a potent anti-neoplastic effect in vitro，but induce unforeseen severe toxicity in vivo［J］. J Control Release，2017，262：212-221.
    [36]
    Katona BW，Anant S，Covey DF，et al. Characterization of enantiomeric bile acid-induced apoptosis in colon cancer cell lines［J］. J Biol Chem，2009，284（5）：3354-3364.
    • Related Articles

  • Related Articles

    [1]YANG Youtian, MAO Baiyang, CHENG Lifei, SU Mengxiang, DI Bin. Identification of the related substances in telmisartan/amlodipine double layer tablets by LC-MS/MS[J]. Journal of China Pharmaceutical University, 2014, 45(1): 65-69. DOI: 10.11665/j.issn.1000-5048.20140111
    [2]CHENG Shou-xian, WANG Rong-hua, CHEN Jie-peng, DUAN Li-li, QIU Xue-lian. Isolation,purification and identification of paclitaxel from the fermented extracts of fungus ST026（Altemaria alternate var.monosporus）[J]. Journal of China Pharmaceutical University, 2011, 42(6): 570-572.
    [3]Study on Chemical Constituents from Breynia fruticosa[J]. Journal of China Pharmaceutical University, 2004, (2): 17-19.
    [4]Release and Identification of Monocrotalic Acid from Dehydromonocrotaline[J]. Journal of China Pharmaceutical University, 2003, (6): 18-21.
    [5]Study on the Constituents of Rabdosia rubescens Hemsl[J]. Journal of China Pharmaceutical University, 2003, (4): 12-14.
    [6]Separation of the Main Impurity P-demethylgatifloxacin from Gatifloxacin and its Synthesis and Identification[J]. Journal of China Pharmaceutical University, 2003, (3): 78-79.
    [7]XU Xiang-Hong, WANG Zheng-Tao, YU Guo-Dian, RUAN Bi-Fang, LI Jun. Alkaloids from Rhizoma corydalis[J]. Journal of China Pharmaceutical University, 2002, (6).
    [8]Isolation and Identification of Two Taxane DiterpenoidCompounds[J]. Journal of China Pharmaceutical University, 2001, (3): 32-34.
    [10]Investigation and Identification of Traditional Chinese Medicinal Materials Aci Pulvis[J]. Journal of China Pharmaceutical University, 1996, (1): 4-5.

  • Cited by

    Periodical cited type(2)

    1. 彭颖，雷晓萌，吴玉欢，夏明艳，魏鑫华，王甜甜，奉建芳，张国松. 维生素A修饰柴胡皂苷a/d脂质体的制备及体内外释药评价. 中国中医基础医学杂志. 2024(10): 1751-1760 .
    2. 李孟孟，何勇，刘扬，徐伟芳，谭松，杨晔，尹登科. 4-甲基肉桂酸小檗碱共晶体外吸收特性、体内药动学研究. 中成药. 2023(05): 1416-1422 .

    Other cited types(0)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(2)
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：365588

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return