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CHENG Xiaohong, JIANG Li, DONG Xingting, KANG Xiulin, LIU Lifang. Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS[J]. Journal of China Pharmaceutical University, 2022, 53(4): 452-466. DOI: 10.11665/j.issn.1000-5048.20220408
Citation: CHENG Xiaohong, JIANG Li, DONG Xingting, KANG Xiulin, LIU Lifang. Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS[J]. Journal of China Pharmaceutical University, 2022, 53(4): 452-466. DOI: 10.11665/j.issn.1000-5048.20220408
shu

Identification of chemical constituents in Fufang Shechuangzi Xiji（Lotion）by UPLC-Q-TOF-MS/MS

  • 1.

    Hospital for Skin Diseases, Chinese Academy of Medical Sciences, Nanjing 210042

  • 2.

    Department of Pharmacognosy, School of Traditional Chinese Pharmacy, China Pharmaceutical University, Nanjing 211198, China

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  • Received Date: March 31, 2022
  • Revised Date: May 26, 2022
    Graphical Abstract
    • Abstract
  • Fufang Shechuangzi Xiji (Lotion) is a common hospital preparation made from clinical prescriptions. Its clinical efficacy is accurate, yet its pharmacodynamic material basis has not been clarified.An ultra-high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS) technique was developed to identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion).The chemical constituents of Fufang Shechuangzi Xiji (Lotion) were scanned by electrospray ionization (ESI) source in positive and negative ion modes.As a result, a total of 118 compounds were identified and characterized via reference standards and by comparing mass spectrometry data with literature, including 45 alkaloids, 21 coumarins and chromones, 19 flavonoids, 14 saponins, 10 anthraquinones and 9 organic acids.As a result, UPLC-QTOF-MS/MS technology can quickly and sensitively identify the chemical constituents of Fufang Shechuangzi Xiji (Lotion), which provides a useful reference for exploring the pharmacodynamic material basis and further quality control study of the preparation.
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    • References (22)
  • [1]
    . J Chin Dermatology（中华皮肤科杂志），1994，27（4）：239-240.
    [2]
    Liu XY，Wu F，Fan GR，et al. Determination of osthol in lotio fructus cnidii Composita by RP-HPLC［J］. Chin Tradit Herb Drugs（中草药），2001，32（9）：25-28.
    [3]
    Zhang Y，Feng BM，Lu X. Research progress on application of UPLC/Q-TOF-MS in pharmaceutical analysis［J］. Nat Prod Res Dev（天然产物研究与开发），2017，29（11）：1992-1996.
    [4]
    Dai WH，Wu LW，Yang SY，et al. Study on antimicrobial activity of alkaoids in Sophora flavescems and S.tonkinensis［J］. Chin J Exp Tradit Med Formulae（中国实验方剂学杂志）， 2012，18（03）：177-180.
    [5]
    Rashid HU，Xu YM，Muhammad Y，et al. Research advances on anticancer activities of matrine and its derivatives：an updated overview［J］. Eur J Med Chem，2019，161：205-238.
    [6]
    Wu ZJ，Sun DM，Fang DM，et al. Analysis of matrine-type alkaloids using ESI-QTOF［J］. Int J Mass Spectrom，2013，341/342：28-33.
    [7]
    Sabatino L，Scarangella M，Lazzaro F，et al. Matrine and oxymatrine in corroborant plant extracts and fertilizers：HPLC/MS-MS method development and single-laboratory validation［J］.J Environ Sci Health B，2015，50（12）：862-870.
    [8]
    Dong YQ，Jia GX，Hu JW，et al. Determination of alkaloids and flavonoids in Sophora flavescens by UHPLC-Q-TOF/MS［J］. J Anal Methods Chem，2021，2021：9915027.
    [9]
    Pilli RA，Rosso GB，de Oliveira M. The chemistry of Stemona alkaloids：an update［J］. Nat Prod Rep，2010，27 （12）：1908-1937.
    [10]
    Wang LX，Wu HW，Liu C，et al. A review of the botany，traditional uses，phytochemistry and pharmacology of Stemonae Radix［J］. Phytochem Rev，2021：1-28.
    [11]
    Zhou Y，Jiang RW，Hon PM，et al. Analyses of Stemona alkaloids in Stemona tuberosa by liquid chromatography/tandem mass spectrometry［J］. Rapid Commun Mass Spectrom，2006，20（6）：1030-1038.
    [12]
    Yang LR，Meng X，Yu XJ，et al. Simultaneous determination of anemoside B4，phellodendrine，berberine，palmatine，obakunone，esculin，esculetin in rat plasma by UPLC-ESI-MS/MS and its application to a comparative pharmacokinetic study in normal and ulcerative colitis rats［J］. J Pharm Biomed Anal，2017，134：43-52.
    [13]
    Liu XW，Zhang F，Gao SH，et al. Metabolite profiling of Zi-Shen pill in rat biological specimens by UPLC-Q-TOF/MS［J］. Chin J Nat Med，2015，13（2）：145-160.
    [14]
    Zhao Q，Li XM，Liu HN，et al. Metabolic map of osthole and its effect on lipids［J］. Xenobiotica，2018，48（3）：285-299.
    [15]
    Gao FY，Hu YS，Ye XL，et al. Optimal extraction and fingerprint analysis of Cnidii fructus by accelerated solvent extraction and high performance liquid chromatographic analysis with photodiode array and mass spectrometry detections［J］. Food Chem，2013，141（3）：1962-1971.
    [16]
    Li J，Ma B，Zhang Q，et al. Simultaneous determination of osthole，bergapten and isopimpinellin in rat plasma and tissues by liquid chromatography-tandem mass spectrometry［J］. J Chromatogr B Analyt Technol Biomed Life Sci，2014，970：77-85.
    [17]
    Xu Y，Yin LL，Wang H，et al. Fragmentation study of flavonoid aglycones with ESI-IT-TOF/MSn in negative ion mode（reviews） ［C］. Report and Proceedings of the 9th National Symposium on Traditional Chinese Medicine and Natural Medicine（第九届全国中药和天然药物学术研讨会大会报告及论文集），2007，46-50.
    [18]
    Matsuda H，Dai Y，Ido Y，et al. Studies on kochiae fructus ⅠV. anti-allergic effects of 70% ethanol extract and its component，momordin Ic from dried fruits of Kochia scoparia L［J］. Biol Pharm Bull，1997，20（11）：1165-1170.
    [19]
    Zhang Q，Qi MD，Kang Y，et al. Rapid identification of glycosides and aglycones in pericarp of Sapindus mukorossi Gaertn.using UHPLC-LTQ orbitrap MS［J］. J Chin Mass Spectrom Soc（质谱学报），2018，39（2）：224-239.
    [20]
    Wen Y，Chen Y，Cui Z，et al. Triterpenoid glycosides from the fruits of Kochia scoparia［J］. Planta Med，1995，61（5）：450-452.
    [21]
    Peng Y，Sun QC，Park Y. The bioactive effects of chicoric acid as a functional food ingredient［J］. J Med Food，2019，22（7）：645-652.
    [22]
    Wang Y，Zhang Q，Xing X，et al. Research progress of mineral medicine Mingfan （Alumen）［J］. J Liaoning Univ TCM（辽宁中医药大学学报），2021，23（10）：17-20.
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