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GU Yunshuang, WANG Rui, SU Na, PENG Ying, A Jiye, WANG Guangji, ZHENG Yiwen, SUN Jianguo. Pharmacokinetic study of Erlong Zuoci Pill in rats[J]. Journal of China Pharmaceutical University, 2022, 53(4): 481-489. DOI: 10.11665/j.issn.1000-5048.20220411
Citation: GU Yunshuang, WANG Rui, SU Na, PENG Ying, A Jiye, WANG Guangji, ZHENG Yiwen, SUN Jianguo. Pharmacokinetic study of Erlong Zuoci Pill in rats[J]. Journal of China Pharmaceutical University, 2022, 53(4): 481-489. DOI: 10.11665/j.issn.1000-5048.20220411
shu

Pharmacokinetic study of Erlong Zuoci Pill in rats

  • 1.

    Jiangsu Provincial Key Laboratory of Drug Metabolism and Pharmacokinetics, Research Unit of PK-PD Based Bioactive Components and Pharmacodynamic Target Discovery of Natural Medicine of Chinese Academy of Medical Sciences, China Pharmaceutical University, Nanjing 210009, China

  • 2.

    Department of Pharmacology and Toxicology, School of Biomedical Sciences, University of Otago, Dunedin 9054, New Zealand

Funds: This study was supported by the National Key R&D Program "Intergovernmental International Cooperation in Science and Technology Innovation" Key Special Funding Project (No.2017YFE0109600) and the Innovation Fund for Medical Sciences of Chinese Academy of Medical Sciences (No.2021-I2M-5-011)
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  • Received Date: April 08, 2022
  • Revised Date: June 13, 2022
    Graphical Abstract
    • Abstract
  • To establish a quantitative LC-MS/MS method for the simultaneous detection of components of Erlong Zuoci Pill in rat plasma: verbascoside, oxypaeoniflorin, echinacoside and benzoylpaeoniflorin, and to evaluate the pharmacokinetic characteristics of Erlong Zuoci Pill in rats, plasma samples were purified by protein precipitation using methanol as a protein precipitant.Methanol was used as the organic phase and aqueous solution containing 0.1% formic acid was used as the water phase.The quantitative analysis method of verbascoside, oxypaeoniflorin, echinacoside and benzoylpaeoniflorin was established in negative ion mode, and the validation of bioanalytical method was carried out.Healthy SD rats were selected, and 20 mL/kg (equivalent to the original drug 10 g/kg dose) of Erlong Zuoci Pill extract was administered by intragastric administration.The plasma concentration of the target compounds at different time intervals after administration was determined using the established method, and the pharmacokinetic parameters was calculated by the Phoenix WinNonlin8.3 software using the non-compartmental model.The method validation results showed that verbascoside (r = 0.993 7) and oxypaeoniflorin (r = 0.994 6) had good linear relationship in the concentration range of 0.5-50 ng/mL, echinacoside (r = 0.993 6) and benzoylpaeoniflorin (r = 0.992 6) had good linear relationship in the concentration range of 1-100 ng/mL.The relative standard deviations of the inter- and intra- batch precision of the four compounds were all less than 15%, and the inter- batch and intra- accuracies were between 85% and 115%.Extraction recovery, matrix effect and stability met the relevant requirements.After a single gavage of Erlong Zuoci Pill extract in rats, all the four compounds were rapidly absorbed and eliminated.Oxypaeoniflorin, echinacoside, and benzoylpaeoniflorin showed two peaks in their drug concentration-time curves.Compared with the other three compounds, oxypaeoniflorin has the highest concentration in rat plasma with cmax1 of (24.40 ± 4.78) ng/mL and cmax2 of (22.50 ± 2.70) ng/mL. The results show that the validation results of this method are in line with the guiding principles of biological sample analysis methods, and it can be used to evaluate the pharmacokinetic characteristics of Erlong Zuoci Pill extract in rats.
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    • References (20)
  • [1]
    . J Audiol Speech Pathol（听力学及言语疾病杂志），2018，26（4）：347-349.
    [2]
    Zhang YB，Yue LY，Yin XY，et al. Effect of tinnitus treatment instrument combined with Ginkgo biloba extract on hearing and hemorheology in patients with sudden deafness and tinnitus［J］. J Otolaryngol Ophthalmol Shandong Univ（山东大学耳鼻喉眼学报），2017，31（5）：50-53，61.
    [3]
    Fan XL，Chen Y，Li L，et al. Efficacy of Chinese herb Cistanche Yishen granules in treatment of tinnitus for patients with chronic nephritis［J］. J Cell Biochem，2019，120（4）：5505-5509.
    [4]
    Huang Y，Fu M. Therapeutic effects of postauricularinjection of lidocaine in tinnitus［J］. J Clin Otorhinolaryngol Head Neck Surg（临床耳鼻咽喉头颈外科杂志），2018（15）：1139-1142.
    [5]
    Pang P，Shi YC，Xu HC，et al. Acupuncture methods put to the test for a tinnitus study：a Bayesian analysis［J］. Complement Ther Med，2019，42：205-213.
    [6]
    Xu P，Yang JY，Lin QP，et al. Factors affecting the efficacy of precision sound therapy in tinnitus treatment［J］. J Audiol Speech Pathol（听力学及言语疾病杂志），2021，29（06）：653-657.
    [7]
    Li XX，Gu CL，Ma YM. Effect of tinnitus consultation combined with multiple compound acoustic therapy on patients with chronic primary［J］. Chin J Otorhinolaryngol Skull Base Surg（中国耳鼻咽喉颅底外科杂志），2020，26（5）：507-511.
    [8]
    Tang DM，Li HW，Chen L. Advances in understanding，diagnosis，and treatment of tinnitus［J］. Adv Exp Med Biol，2019，1130：109-128.
    [9]
    Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia：part 1（中华人民共和国药典：一部）［S］.Beijing：China Medical Science Press，2020：865.
    [10]
    Wu Y，Wang Z，Ma XD，et al. Effect of Erlong Zuoci pill on expression levels of cAMP and PKA in tissue of mice with presbycusis［J］. Chin Arch Tradit Chin Med（中华中医药学刊），2017，35（6）：1548-1551.
    [11]
    Xie J，Zheng TH. Effect of Erlongzuoci pill and Fucong Tongmai Kaiqiao acupuncture in treating nervous deafness and tinnitus and its influence on serum NO and Connexin 26［J］. Mod J Integr Tradit Chin West Med（现代中西医结合杂志），2017，26（33）：3671-3673.
    [12]
    Wang J，Guo CR，Dong Y，et al. Effects of Erlong Zuoci pills and its effective disassembled prescriptions on gentamycin induced hair cell apoptosis［J］. China J Chin Mater Med（中国中药杂志），2010，35（18）：2464-2468.
    [13]
    Gong YC，Li N，Wang YR，et al. Effects of Erlong Zuoci Pills on autophagy and apoptosis of HEI-OC1 cells by regulating miR-34a［J］. China J Tradit Chin Med Pharm（中华中医药杂志），2022，37（2）：740-744.
    [14]
    Huo SX，Li JM，Gao L，et al. Absorption，distribution and excretion of acteoside in rats［J］. Chin J Hosp Pharm（中国医院药学杂志），2016，36（6）：450-454.
    [15]
    Hu Y，Gao JX，Ji Z，et al. Pharmacokinetics of four active estrogenic constituents in total glucosides of Cistanche deserticola［J］. Chin Tradit Pat Med（中成药），2021，43（8）：1988-1994.
    [16]
    Chen XZ，Ma HL，Ding LQ，et al. Simultaneous determination of main monoterpenoids in radix paeoniae rubra extract by UPLC-MS/MS method and analysis of their pharmacokinetics in plasma of rats ［J］.Liaoning J Tradit Chin Med（辽宁中医杂志）2018，45（3）：590-595.
    [17]
    Cheng YS，Peng C，Wen FY，et al. Pharmacokinetic comparisons of typical constituents in white peony root and sulfur fumigated white peony root after oral administration to mice［J］. J Ethnopharmacol，2010，129（2）：167-173.
    [18]
    Shen JY，Yang XL，Yang ZL，et al. Enhancement of absorption and bioavailability of echinacoside by verapamil or clove oil［J］. Drug Des Devel Ther，2015，9：4685-4693.
    [19]
    Feng C，Liu M，Shi XW，et al. Pharmacokinetic properties of paeoniflorin，albiflorin and oxypaeoniflorin after oral gavage of extracts of Radix Paeoniae Rubra and Radix Paeoniae Alba in rats［J］. J Ethnopharmacol，2010，130（2）：407-413.
    [20]
    Zhou XW，Bao ZZ. A pharmacokinetic study of benzoyl paeoniflorin in rats ［J］. Jiangsu J Tradit Chin Med（江苏中医药），2019，51（2）：80-82.
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