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SHI Xiaolu, CHE Xinfeng, WU Jianmei, DI Bin, QIAO Hongwei, WANG Youmei. Evaluation of the cut-off value of methamphetamine,amphetamine,6-monoacetylmorphine, and morphine in hair[J]. Journal of China Pharmaceutical University, 2022, 53(5): 554-562. DOI: 10.11665/j.issn.1000-5048.20220506
Citation: SHI Xiaolu, CHE Xinfeng, WU Jianmei, DI Bin, QIAO Hongwei, WANG Youmei. Evaluation of the cut-off value of methamphetamine,amphetamine,6-monoacetylmorphine, and morphine in hair[J]. Journal of China Pharmaceutical University, 2022, 53(5): 554-562. DOI: 10.11665/j.issn.1000-5048.20220506
shu

Evaluation of the cut-off value of methamphetamine,amphetamine,6-monoacetylmorphine, and morphine in hair

  • 1.

    School of Pharmacy, China Pharmaceutical University, Nanjing 210009

  • 2.

    Office of China National Narcotics Control Commission-China Pharmaceutical University Joint Laboratory on Key Technologies of Narcotics Control, Beijing 100193

  • 3.

    Key Laboratory of Drug Monitoring and Control, Drug Intelligence and Forensic Center, Ministry of Public Security, Beijing 100193, China

Funds: This study was supported by the Special Project of Basic Work of Strengthening Police by Science and Technology of the Ministry of Public Security (No.2020GABJC22)
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  • Received Date: June 06, 2022
  • Revised Date: September 08, 2022
    Graphical Abstract
    • Abstract
  • A rapid determination of methamphetamine, amphetamine, 6-monoacetylmorphine, and morphine in hair samples by UPLC-MS/MS was established and optimized.The concentration of target compounds in the hair of drug abusers and drug laboratory technicians was investigated and the cut-off value was evaluated.After cleaned hair was extracted by grinding with methanol-water (7∶3) at 3 000 r/min for 100 s, the final solution after adjusting the volume to methanol-water (1∶1) was analyzed by UPLC-MS/MS.The analytes were gradient eluted on a Waters Acquity BEH C18 (2.1 mm × 100 mm, 1.7 μm) column with 5 mmol/L ammonium formate-0.1% formic acid aqueous solution and acetonitrile as mobile phase at a flow rate of 0.4 mL/min. The ESI+ ion source and multiple reaction monitoring (MRM) were used to select the qualitative and quantitative ion pairs of the four target compounds. All analytes showed good linearity (R2 ≥ 0.999 6) in the range of 0.01-5 ng/mg (except amphetamine in 0.01-4 ng/mg), limit of the quantitation was 0.01 ng/mg, and the limit of detection was 0.001-0.008 ng/mg.The accuracy, precision, matrix effect, and recovery all met the requirements of biological sample methodology.According to the comprehensive consideration of the receiver operating characteristic (ROC) curve, Youden index, law enforcement cost and intensity, the reference cut-off values were methamphetamine ≥ 0.1 ng/mg; amphetamine ≥ 0.025 ng/mg; 6-monoacetylmorphine ≥ 0.05 ng/mg; morphine ≥ 0.05 ng/mg.The method established in our research can quickly and accurately detect the contents of methamphetamine, amphetamine, 6-monoacetylmorphine, and morphine in hair.This study provides some reference for the public security system to make more rational cut-off values in the norm of drug-related personnel hair samples detection in the future.
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