Progress of proximity labeling technology in membrane protein interaction

PEI Xin; LI Guideng; CHU Weihua

doi:10.11665/j.issn.1000-5048.2023041303

Journal of China Pharmaceutical University > 2024 > 55(2): 158-166. > DOI: 10.11665/j.issn.1000-5048.2023041303

PEI Xin, LI Guideng, CHU Weihua. Progress of proximity labeling technology in membrane protein interaction[J]. J China Pharm Univ, 2024, 55(2): 158 − 166. DOI: 10.11665/j.issn.1000-5048.2023041303

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Progress of proximity labeling technology in membrane protein interaction

PEI Xin^{1, 2,},
LI Guideng^2, ,,
CHU Weihua^1, ,

1.
School of Life Science and Technology, China Pharmaceutical University, Nanjing 211198
2.
Suzhou Institute of Systems Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, Suzhou 215123, China

Funds: This work was supported by the National Natural Science Foundation of China (No. 81972875，No.32270994) and the Natural Science Foundation Outstanding Youth Fund of Jiangsu Province (No. BK20211505)

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Received Date: April 12, 2023

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Abstract

Abstract

Membrane proteins, which play a critical role in various life processes, particularly in regulating cell-cell contact and signal transduction, are closely linked to cell differentiation and maturation. Therefore, it is of great theoretical and practical significance to develop a variety of methods to thoroughly explore the interactions between membrane proteins. In addition to traditional techniques such as immunoprecipitation, newly developed proximity labeling (PL) techniques have gradually become important means to study membrane protein interaction. PL methods are based on engineered enzymes fused with bait protein to catalyze small molecules, label neighboring target proteins, and detect the interactions by flow cytometry, mass spectrometry, confocal microscopic imaging, etc. This paper focuses on the recent developments in PL techniques for studying membrane protein interactions, with a prospect of the potential future directions for research in this area.
- proximity labeling,
- cell membrane protein,
- membrane protein interaction,
- engineered enzyme

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References (61)

References

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Progress of proximity labeling technology in membrane protein interaction