UV Spectrophotometric and Colorimetric Determination of Ortho Dihydric phenolic Drugs Ⅳ. Determination of Isoprenaline Hydrochloride and It's Content in Aerosol

The ultraviolet spectrophotometric method and colorimetric method are used for thedetermination of isoprenaline hydrochlorid aerosol. The former is carried out bymeasuring absorbance at 279nm±mm. The absorption coefficient of isoprenaline isdetermined,E_(1cm)~(1%)279nm=112.8; The latter method is based on the reaction ofisoprenaline with sodium nitrite-sodium molybdate at alkaline medium forming a redcompound. By measuring the absorption of such red compound at 500±mm, it ispossible to determine the isoprenaline content in the sample. The concentration rangeof isoprenaline is between 4～40μg/ml, which is in agreement with Beer's laws.Theisoprenaline in the aerosol is also determined colorimetrically. Interferences to owning guaiphenesin and ascorbic acid are negligible.