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Dai Dezai, Zhang Hui, Wang Yuzu, Louise Loumikounov. EFFECT OF TEMPERATURE ON FLUOROMETRIC ASSAY OF 5-HYDROXYLTRYPTAMINE AND 5-HYDROXYLINDOLE ACETIC ACID[J]. Journal of China Pharmaceutical University, 1983, (2): 65-66.
Citation: Dai Dezai, Zhang Hui, Wang Yuzu, Louise Loumikounov. EFFECT OF TEMPERATURE ON FLUOROMETRIC ASSAY OF 5-HYDROXYLTRYPTAMINE AND 5-HYDROXYLINDOLE ACETIC ACID[J]. Journal of China Pharmaceutical University, 1983, (2): 65-66.
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EFFECT OF TEMPERATURE ON FLUOROMETRIC ASSAY OF 5-HYDROXYLTRYPTAMINE AND 5-HYDROXYLINDOLE ACETIC ACID

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  • The optimal temperature for developing fluorometric readings in estimating 5—hydroxyltryptamine (5-HT) and 5—hydroxylindole acetic acid (5—HIAA) is found to be 80℃. The sensitivity of the assay is sharply declined when temperature is raised up to 100℃ particularly in estimating 5—HIAA.
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