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Dong Shanshi, Fan Ming, An Dengkui. HPLC DETERMINATION OF PILOCARPINE AND ITS DEGRADATION PRODUCTS IN OPHTHALMIC FILMS[J]. Journal of China Pharmaceutical University, 1984, (1): 17-22.
Citation: Dong Shanshi, Fan Ming, An Dengkui. HPLC DETERMINATION OF PILOCARPINE AND ITS DEGRADATION PRODUCTS IN OPHTHALMIC FILMS[J]. Journal of China Pharmaceutical University, 1984, (1): 17-22.
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HPLC DETERMINATION OF PILOCARPINE AND ITS DEGRADATION PRODUCTS IN OPHTHALMIC FILMS

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    • Abstract
  • A high—performance liquid Chromatographic method was developed to determine pilocarpine and its degradation products, isopilocarpine and pilocarpine acid in ophthalmic films. An ion—exchange resin ( Fartisil-lOSCX ) column was employed with 0.3 M phosphate buffer ( pH 6 ) containing 5% isopropanol as mobile phase. The influence of ionic concentration and acidity on column-performance was also investigated. The average recovery of five samples was found to be 100.3±0.6%.
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