The Construction of an E.coli Strain with Expression of Fumarate Hydratase

An 1.8 kb fumA fragment,amplified out from E.coli chromosome DNA by PCR technology,was inserted into plasmid pUC19.The recombinant plasmid was used to transform E.coli JM105.From the transformants,a strain, named CPU-W-9211 was obtained,whose activity of