Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli

kb DNA fragment was obtained from E. coli JM 105 by PCR. This fragment was inserted into NdeI/BamHI sites of pET3a. The recombinant plasmids were transformed into host strain E. coli BL 21(DE3). High level expression of tryptophanase genetic engi