Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli

Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli[J]. Journal of China Pharmaceutical University, 1999, (2): 61-64.

Citation:

Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli[J]. Journal of China Pharmaceutical University, 1999, (2): 61-64.

Citation:

Molecular Cloning and Expression of Tryptophanase Gene of Escherichia coli[J]. Journal of China Pharmaceutical University, 1999, (2): 61-64.

Abstract

kb DNA fragment was obtained from E. coli JM 105 by PCR. This fragment was inserted into NdeI/BamHI sites of pET3a. The recombinant plasmids were transformed into host strain E. coli BL 21(DE3). High level expression of tryptophanase genetic engi

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