ConstitutiveHigh-Expression of the N-Acetylneuraminate Lyase Gene of Escherichia coli

bp DNA fragment encoded N acetylneuraminate lyase was amplified from E.coli JM 105 by PCR. This fragment was inserted into NcoI/BamHI sites of pET28a. The recombinant plasmids were transformed into host strain E.coli BL 21(DE3). High level expre