Advanced Search
Cloning of Spinach Glycolate Oxidase cDNA and Its Construction into Yeast Expression Vector[J]. Journal of China Pharmaceutical University, 2003, (1): 83-86.
Citation: Cloning of Spinach Glycolate Oxidase cDNA and Its Construction into Yeast Expression Vector[J]. Journal of China Pharmaceutical University, 2003, (1): 83-86.

Cloning of Spinach Glycolate Oxidase cDNA and Its Construction into Yeast Expression Vector

More Information
  • METHOD:Using spinach to extract total RNA, the spinach GO cDNA was amplified by RT PCR. This cDNA was inserted into cloning vector pMD T and sequenced. The cDNA was then cloned into pPIC3 5K(P.Pastoris expression vector)by SnaBI/NotI double digestion.
  • Cited by

    Periodical cited type(3)

    1. 任萌,刘菲,张辉,杨素珍,焦雅培,王先浩,苏蓓,李晓彤. 透明质酸的性能及其载体化应用. 山东化工. 2024(10): 112-117+120 .
    2. 侯效英,高帆,杜丽东,王瑞琼,吴国泰. 藁本内酯稳定性研究进展. 中国药学杂志. 2024(24): 2299-2305 .
    3. 高雷雷,王芳,陈艳君,王威,李国四,韩邦兴,刘东. OBE教育理念下药物化学实验设计:双丹皮酚缩1, 3-丙二胺席夫碱的合成. 广东化工. 2023(24): 145-147 .

    Other cited types(1)

Catalog

    Article views (1225) PDF downloads (836) Cited by(4)
    Turn off MathJax
    Article Contents

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return