Isolation and Purification of Recombinant Hirudin-2 From Fermentation Medium
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Graphical Abstract
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Abstract
AIM:The purpose is to establish the isolation and purification process of recombinant hirudin-2(rHV 2 ) expressed in pichia. METHOD: Recombinant pichia was cultured with high cell density and induced to express rHV 2 to culture medium,rHV 2 was
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