Cellular uptake characteristics of salvianolic acid B in myocardial cells and blood vessel endothelial cells

Aim ：To determine the uptake characteristics of salvianolic acid B(Sal B) in myocardial cells and blood vessel endothelial cells. Method ：The effects of various factors，such as time，temperature，drug concentration，pH of the medium，on the uptake of Sal B in myocardial cells and aorta endothelial cells were investigated.LC/MS was employed to determine the intracellular concentration of Sal B. Results ：Uptake kinetics of Sal B in the myocardial cells and aorta endothelial cells fitted well to the logarithmic model at 37 ℃ and 4 ℃.The amount of uptake was in direct proportion to the extracellular concentration of Sal B in the experimental concentration range.Uptake of Sal B both in the myocardial cells and blood vessel endothelial cells would significantly increase while the medium pH decreased，and some water-soluble components extracted from danshen would also facilitate the uptake of Sal B both in the myocardial cells and blood vessel endothelial cells obviously.The energy metabolism inhibitors would significantly inhibit the uptake of Sal B in the myocardial cells and blood vessel endothelial cells.When lactic acid and fatty acid were added to the incubation solution，the uptake of Sal B both in the myocardial cells and aorta endothelial cells increased more than 20%. Conclusion ：pH is the most important factor influencing the cellular uptake of Sal B，and the amount of uptake tends to increase in acidic medium.Results suggest that the uptake of Sal B would increase in the acidified internal environment induced by myocardial ischemia，thus exerting better cardiovascular activities.