Preparation and application of immobilized chiral stationary phase bonded cellulose

Aim ：To prepare the immobilized 3，5-dichlorophenylcarbamate of cellulose on allylsilica gel as chiral stationary phase，and to study its application on chiral drug analysis. Methods ：10-Undecenoate/3，5-dichlorophenylcarbamate derivatives of cellulose was synthesized and efficiently immobilized on allylsilica gel by radical polymerization reaction. The chiral stationary phase (CSP) was evaluated by HPLC separation of enantiomers. The influence on separation resulted by the composition of mobile phase was discussed. Results ：Four enantiomers including praziquantel were separated. Conclusion ：The self made column has high solvent resistance.