Synthesis of N-acyl chitosan derivatives and investigation of chitosan-modified docetaxel liposomes in vitro

Aim ：Synthesized N-acylated chitosan(NAC) was used to anchor on the surface of plain docetaxel liposomes(PDL) in order to to sustain drug release. Methods ；NAC of low substitution degrees were prepared using hexanoic(C₆)，lauric(C₁₂)，and palmitic(C₁₆) anhydrides.N-palmitoyl chitosan was chosen to anchor the docetaxel liposomes.In vitro release profiles of conventional liposmes and the anchored liposomes was compared. Results ：Hexanoic(C₆)，lauric(C₁₂)，and palmitic(C₁₆)-branched chitosans were synthesized.It was found that N-acylated chitosan-anchored DXL liposomes(NDL) increase stabilities of docetaxel liposomes.70% of docetaxel was released from PDL in 24 hours but 39% from NDL. Conclusion ：Liposomes anchored by the low substituted N/-acylated chitosan could decrease the drug release.