Toxicity of glufosfamide in Beagle dogs following intravenous injection

Aim ：To evaluate the safety of glufosfamide in Beagle dogs. Methods ：The safety of glufosfamide in Beagle dogs following single iv and multiple iv administration for 3 months was observed. Results ：The minimum lethal dose of glufosfamide was 100 mg/kg.The maximum non-lethal dose of glufosfamide was 50 mg/kg.The approximate lethal dose of glufosfamide was 75-100 mg/kg.No serious adverse reactions was observed in Beagle dogs following multiple iv administration once a week for 3 months at the dose of 20 mg/kg，40 mg/kg，and 60 mg/kg.However，organ injury was observed extensively in the high dose-group and mediumt-dose group.The low-dose group showed a light change in individual organs. Conclusion ：Under the experimental conditions，40 mg/kg and 60 mg/kg iv administration once a week for 3 months can lead to extensive organ injury in Beagle dogs，so the dose shouldn′t be higher than 20 mg/kg.