Preliminary structural identification and protection on renal cell injury of acidic polysaccharide from Cordyceps sinensis

Aim ：To elucidate the structure of an acidic polysaccharide from Cordyceps sinensis and to study its protective effect on renal cell injury by cisplatin. Methods ：After Cordyceps sinensis acidic polysaccharide(CAPS) was isolated and purified，the purity and molecule weight were detected by high performance gel permeation chromatography(HPGPC).Based on the UV spectrum，FT-IR spectrum，monosaccharide composition and GC-MS，the structures were elucidated.Cisplatin-induced injury of Madin-Darby canine kidney（MDCK） cell line was protected by the blood serum with CAPS. Results ：The purity of CAPS，with a molecule weight of 2.7×10⁴，was about 99%.It consisted mainly of mannose，glucose and galactose.When the serum with CAPS reached 2.5%，it showed difference compared with the cisplatin group(P＜0.05) on the protective effect of renal cell injury.It showed significant difference(P＜0.01) when the serum with CAPS exceeded 5%. Conclusion ：CAPS has a protective effect on the renal cell injury in vitro.