Computer-aided design of the 10-23 deoxyribozyme targeting resistance gene mecR1

The purpose of this study was to exploit the potential of Primer Premier 5.0 and RNA structure 4.6 in the design and selection of effective 10-23 deoxyribozyme (DRz) targeting resistance gene mecR1.Five designed and synthesized anti-mecR1 10-23 DRz sequences were introduced into the MRSA strain by electro transformation in vivo.Transcription of mecR1 was analyzed by real-time quantitative PCR.The inhibitory effects of DRzs on the bacterial growth were evaluated based on the plate cloning formation.It was found that the five anti-mecR1 10-23 DRz sequences significantly lowered the transcription of mecR1 and inhibited the growth of clinical drug-resistant MRSA080309，with DRz6 having the most significant inhibitory effect.Therefore，the combination of the two computer softwares is an economical，practical and effective approach in the design of anti-mecR1 DRz，and could greatly reduce the screening time of antisense drugs.