Preparation and application of affinity chromatography medium with Ginsenoside Rg1 as a ligand

Ginsenoside Rg1(Rg1) is one major active component of the traditional Chinese herb，Ginseng.It showed remarkable neuroprotective effects in vitro and in vivo.In order to provide new technological support for the identification of the target of Rg1 and further explanation of its activities，the affinity medium Rg1-epoxy-activated sepharose^TM6B(EAS6B) was prepared by coupling Rg1 to EAS6B.The copulation was optimized by a L₉₍)(3⁴) orthogonal design experiment，and verified by IR.The optimal coupling method was determined as 40 ℃，pH 13，n(Rg1)∶V(EAS6B)=100 μmoL∶1 mL according to the orthogonal analysis and practical application.The coupling rate and yield were 31.47% and 31.56 μmol/mL in amplified preparation.Furthermore，a specific protein band was found from PC12 lysate using Rg1-EAS6B medium by serial affinity chromatography from PC12 cell lysate.The study may provide some references for further identifying the target of Rg1 and preparing affinity chromatography resin of other natural compounds with similar active group.