Robustness test on determination of luteolin-7- O-glucoside in Flos Lonicerae

In accordance with the Chinese Pharmacopoeia,chromatographic separation conditions were studied to determine luteolin-7-O-glucoside in Flos Lonicerae under different RP-HPLC chromatographic conditions.Results showed that when a YMC-Pack ODS-A column was adopted with the gradient elution using the mobile phase of acetonitrile as mobile phase A,0.5% acetic acid solution as mobile phase B, the detection wavelength was 350 nm,the flow rate was 1 mL/min, and the column temperature was 40 °C, the better separation, precision and repeatability were achieved. It could meet the relevant requirements of the Chinese Pharmacopoeia; and the chromatographic separation conditions could be used as the conditions for the determination of luteolin-7-O-glucoside in Flos Lonicerae.