Effect of content variation of Danshen water-soluble components on myocardial energy metabolism in isolated ischemia-reperfusion rat hearts

To investigate the effect of content variation of water-soluble components in Danshen injection on the myocardial energy metabolism in the isolated ischemia/reperfusion rat hearts.Rats were randomly divided into model groups,Danshen injection group and four component-adding groups.Drugs were administered when myocardial ischemia/reperfusion injury occurred,and the pharmacodynamics indices such as acetyl coenzyme A(AcCoA),coenzyme A(CoA),adenine nucleotides(AMP,ADP and ATP) and myocardial ischemia markers (lactate (LD),lactate dehydrogenase(LDH) and creatine kinase(CK)) were determined.Compared with the control group,the AcCoA/CoA ratio decreased significantly after adding protocatechuic acid (PA) (P<0.05),while the total adenosine (TAN) and adenylate energy charge (AEC) increased obviously (P<0.05).The effect of adding salvianolic acid B (SalB) was similar to PA,but the regulatory effect on the energy metabolism was weaker in total.A back-propagation neural network model was developed using experimental data,and the contribution of the four water-soluble components’contents to the pharmacodynamic indices were evaluated according to Garson algorithm.The effect of various components on the fatty acids and high-energy phosphate compounds ranked as PA>SalB>SalA>DSS;and the impact of components on the myocardial ischemia markers could be put in the following order:PA>SalB>DSS>SalA.The results suggest that water-soluble components of Danshen injection could save myocardial ATP and increase AEC,meanwhile,the AcCoA/CoA ratio in the tissue was reduced,thereby improving the efficiency of myocardial energy metabolism,during which the phenolic acids showed significant effects.