Purification and clotting activity of a thrombin-like enzyme from Zhejiang agkistrodon acutus venom

A thrombin-like enzyme named TQ-1 was purified by column chromatography from Zhejiang agkistrodon acutus venom.The structure of TQ-1 was studied by RP-HPLC,Q-TOF,SDS-PAGE,Edman degradation and isoelectric focus;clotting activity of TQ-1 was studied in vivo and vitro.The purity of TQ-1 by was 98.5%,the molecular weight was 30 522.95,the isoelectric point was 4.5,the N-terminal amino acids sequence was VIGGNECDTNEHRFL,the specific activity in vitro was 900U/mg,and the clotting activity in vivo was similar to that of the positive control.TQ-1 is a new type of thrombin-like enzyme with a high medicinal value.