Relationship between ingredient of polysorbate 80 and its hemolysis

The relationship between hemolysis and the structure of polysorbate 80(Tween 80) was investigated in this paper.Three different products obtained in the first step of synthesis process of Tween 80 are sorbitol,sorbitan and isosorbide,which could form the mixture after esterification and etherification.The ingredients and the hemolysis rates of polyoxyethylene sorbitol oleate(POS) and polyoxyethylene isosorbide oleate(POI) were detected by MALDI-TOF-MS and hemolysis testing.The results showed that POS has an extremely low hemolysis rate while POI had a higher rate.The conclusion was further proved by extraction method.