In vitro antitumor effect of the sulfobutyl-β-cyclodextrin inclusion of docetaxel for injection

To evaluate the in vitro cytotoxicity of the sulfobutyl-β-cyclodextrin inclusion of docetaxel for injection (ML061) on various tumor cell lines,14 tumor cell lines including MDA-MB-435,MDA-MB-435s,NCI-H446,HO-8910,Bcap-37,KB,SMMC-7721,HT-29,SW480,LS-174t,SGC-7901,MCF-7,A549 and NCI-H460 were used for the treatment with ML061 at different concentrations (200,20,2,0.2,0.02,0.002,0.000 2,0.000 02 μg/mL) for 72 h,then the cell proliferation was evaluated by MTT method,and finally IC₅₀ was calculated.It was found that ML061 selectively inhibited the proliferation of a variety of tumor cell lines in vitro,with a certain in vitro killing effect on the 14 tumor cell lines in a dose-dependent trend.It was found that among the 14 tumor cell lines,the IC₅₀ values of Bcap-37,MDA-MB-435,MDA-MB-435s,NCI-H446 and SW480 were less than 0.1 μg/mL(being sensitive to ML061).Compared with the positive control Aomingrun,ML061 had stronger in vitro inhibition effect on human ovarian cancer Bcap-37,human colon cancer SW480,human small cell lung cancer NCI-H446,human breast cancer MDA-MB-435 and MDA-MB- 435s.In conclusion,ML061 inhibits the proliferation of a variety of tumor cell lines in vitro,of which Bcap-37,SW480,NCI-H446 and MDA-MB-435s cell lines are as sensitive as Aomingrun,but MDA-MB-435 is more sensitive to ML061 than Aomingrun.