Simultaneous determination of seven compounds in Eucommiae Folium by HPLC

A high-performance liquid chromatographic method was established to evaluate the quality of Eucommiae Folium with a simultaneous determination of seven compounds,chlorogenic acid,quercetin 3-O-α-L-arabinopyranosyl-(1→2)-β- D-glucopyranoside,rutin,isoquercitrin,astragaline,quercetin and kaempferol.The samples were separated on a Hedera-C₁₈ column with gradient elution of acetonitrile and 0.1% methanoic acid at a flow rate of 1.0 mL/min and detected at 270 nm.The seven compounds were completely separated within 45 min and the determinations indicated good linearity.The average recoveries varied from 99.03% to 107.05%.This method was validated with respect to precision,repeatability and accuracy,and was successfully applied to the quantification of seven compounds in 10 commercial samples of Eucommia ulmoides Oliv..