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LIU Yongli, ZHAO Zhenxia, LI Dongmei, FENG Li. Simultaneous determination of seven components in Huoxiang Zhengqi liquid by ultra-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2013, 44(3): 249-252. DOI: 10.11665/j.issn.1000-5048.20130312
Citation: LIU Yongli, ZHAO Zhenxia, LI Dongmei, FENG Li. Simultaneous determination of seven components in Huoxiang Zhengqi liquid by ultra-performance liquid chromatography[J]. Journal of China Pharmaceutical University, 2013, 44(3): 249-252. DOI: 10.11665/j.issn.1000-5048.20130312
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Simultaneous determination of seven components in Huoxiang Zhengqi liquid by ultra-performance liquid chromatography

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  • An ultra-performance liquid chromatographic method was established for the simultaneous determination of seven active compounds in Huoxiang Zhengqi liquid. Seven compounds were analyzed simultaneously with an Acquity BEH C18 column by gradient elution using acetonitrile-0. 1% phosphoric acid as the mobile phase. The column was maintained at 35 °C; the flow rate was 0. 4 mL/min; the detection wavelengths were set at 249, 284 and 336 nm, respectively. Good linearity(r> 0. 999 9)in the range of the test concentration was observed between concentration and chromatographic response of each compound, and the average recoveries of the method were between 96. 16% and 103. 4%.
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