Preparation and characterization of a self-assembled tea polyphenol-gelatin-chitosan nanoparticles

ZHANG Yin; MENG Chen; CHANG Jun; SHA Sha; CHEN Hao; LU Suo

doi:10.11665/j.issn.1000-5048.20140209

Journal of China Pharmaceutical University > 2014 > 45(2): 178-184. > DOI: 10.11665/j.issn.1000-5048.20140209

ZHANG Yin, MENG Chen, CHANG Jun, SHA Sha, CHEN Hao, LU Suo. Preparation and characterization of a self-assembled tea polyphenol-gelatin-chitosan nanoparticles[J]. Journal of China Pharmaceutical University, 2014, 45(2): 178-184. DOI: 10.11665/j.issn.1000-5048.20140209

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Preparation and characterization of a self-assembled tea polyphenol-gelatin-chitosan nanoparticles

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A kind of self-assembled tea polyphenol(TP), gelatin(Gel)and chitosan(Cs)nanoparticles(TP-Gel-Cs)was prepared and evaluated in vitro. TP-Gel-Cs nanoparticles were fabricated based on an electrostatic bonding between Gel and Cs, together with a non-covalent interaction between TP and polysaccharide modified Gel. The optimal formulation of nanoparticles was a mass ratio of 1. 5 ∶3 ∶1 for TP-Gel-Cs nanoparticles. The present experiments showed that the primary interaction exiting within the TP-Gel-Cs matrix was non-covalent bond. The mean diameter of the particles was 971. 50 nm with a polydispersity index of 0. 22, the Zeta-potential was -10. 44 mV at the neutral condition and +33. 23 mV at pH 5. 4. The entrapment efficiency and loading capacity of the particles were 90. 42% and 26. 82%, respectively. The stability of TP at 25 ℃ was significantly improved by formation of TP-Gel-Cs nanoparticles, the nanoparticles exhibit both pH-sensitive and controlled release properties. Taken together, the preparation process is simple, mild and green, which may be a promising method to encapsulation polyphenol substances.
- tea polyphenol,
- gelatin,
- chitosan,
- nanoparticles,
- self-assembly

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[1]	Dube A,Ng K,Nicolazzo JA,et al.Effective use of reducing agents and nanoparticle encapsulation in stabilizing catechins in alkaline solution[J].Food Chem,2010,122(3):662-667.
[2]	Bao SB,Xu SY,Wang Z.Antioxidant activity and properties of gelatin films incorporated with tea polyphenol-loaded chitosan nanoparticles[J].J Sci Food Agric,2009,89(15):2 692-2 700.
[3]	Zhang LY,Kosaraju SL.Biopolymeric delivery system for controlled release of polyphenolic antioxidants[J].Eur Polym J,2007,43(7):2 956-2 966.
[4]	Shpigelmn A,Israeli G,Livney YD.Thermally-induced protein-polyphenol co-assemblies:beta lactoglobulin-based nanocomplexes as protective nanovehicles for EGCG[J].Food Hydrocolloid,2010,24(8):735-743.
[5]	Siddiqui IA,Mukhtar H.Nanochemoprevention by bioactive food components:a perspective[J].Pharm Res,2010,27(6):1 054-1 060.
[6]	Whitesides G M,Grzybowski B.Self-assembly at all scales[J].Science,2002,295(5 564):2 418-2 421.
[7]	Kim SW,Nimni ME,Yang Z,et al.Chitosan/Gelatin-based films crosslinked by proanthocyanidin[J].J Biomed Mater Res Appl Biomater,2005,75(2):442-450.
[8]	Zou T,Percival SS,Cheng Q,et al.Preparation,characterization,and induction of cell apoptosis of cocoa procyanidins-gelatin-chitosan nanoparticles[J].Eur J Pharm Biopharm,2012,82(1):36-42.
[9]	Shutava TG,Balkundi SS,Vangala P,et al.Layer-by-layer-coated gelatin nanoparticles as a vehicle for delivery of natural polyphenols[J].ACS Nano,2009,3(7):1 877-1 885.
[10]	Zhou HH,Sun XY,Zhang LL,et al.Fabrication of biopolymeric complex coacervation core micelles for efficient tea polyphenol delivery via a green process[J].Langmuir,2012,28(41):14 553-14 561.
[11]	Chen YC,Yu SH,Tsai GJ,et al.Novel technology for the preparation of self-assembled catechin/gelatin nanoparticles and the characterization[J].J Agric Food Chem,2010,58(11):6 728-6 734.
[12]	Jöbstl E,O′Connell J,Fairclough JPA,et al.Molecular model for astringency produced by polyphenol/protein interactions[J].Biomacromolecules,2004,5(3):942-949.
[13]	Frazier RA,Deaville ER,Green RJ,et al.Interactions of tea tannins and condensed tannins with proteins[J].J Pharm Biomed,2010,51(2):490-495.
[14]	Zhang LY,Dudhani A,Lundin L,et al.Macromolecular conjugate based particulates:preparation,characterisation and evaluation of controlled release properties[J].Eur Polym J,2009,45(7):1 960-1 969.
[15]	Pascal C,Poncet-Legrand C,Cabane B,et al.Aggregation of a proline-rich protein induced by epigallocatechin gallate and condensed tannins:effect of protein glycosylation[J].J Agric Food Chem,2008,56(15):6 724-6 732.
[16]	Li KG,Wang Y,Miao ZC,et al.Chitoan/gelatin composite microcarrier for hepatocyte culture[J].Biotechnol Lett,2004,26(11):879-883.
[17]	Takeuchi H,Yasuji T,Yamamoto H,et al.Spray-dried lactose composite particles containing an ion complex of alginate-chitosan for designing a dry-coated tablet having a time-controlled releasing function[J].Pharm Res,2000,17(1):94-99.
[18]	Liu ZY,Ge XJ,Lu Y,et al.Effects of chitosan molecular weight and degree of deacetylation on the properties of gelatine-based films[J].Food Hydrocolloid,2012,26(1):311-317.
[19]	Song H.Pharmaceutical Engineering Specialty Experiment(制药工程专业实验)[M].Beijing:Chemical IndustryPublishing House,2005:151-152.
[20]	Lv Y.Perparation of jasmine essence micro/nano-capsule via complex coacervation and its mechanism research(复合凝聚反应制备茉莉香精微/纳米胶囊及其机理研究)[D].Wuxi:Jiangnan University,2012.
[21]	Strauss G,Gibson SM.Plant phenolics as cross-linkers of gelatin gels and gelatin-based coacervates for use as food ingredients[J].Food Hydrocolloid,2004,18(1):81-89.
[22]	Xing FB.Study on the microcapsules and nanocapsules of gelatin-acacia-tannin containing capsaicin(辣素/明胶-阿拉伯胶-单宁微囊和纳囊的研究)[D].Tianjin:Tianjin University,2003.
[23]	Passcal C, Poncet-Legrand C, Imberty A, et al. Interaction between a non glycosylated human proline-rich protein and flavan-3-ols are affected by protein concentration and polyphenol/protein ratio[J].J Agric Food Chem,2007,55(12):4 895-4 901.
[24]	Bao SB.Study on gelatin films incorporated with tea polyphenol loaded nanoparticles(茶多酚纳米粒复合明胶膜的研究)[D].Wuxi:Jiangnan University,2009.
[25]	Dudhani AR,Kosaraju SL.Bioadhesive chitosan nanoparticles:preparation and characterization[J].Carbohydr Polym,2010,81(2):243-251.
[26]	Chen TH,Embree HD,Brown EM,et al.Enzyme-catalyzed gel formation of gelatin and chitosan:potential for in situ applications[J].Biomaterials,2003,24(17):2 831-2 841.
[27]	Rocasalbas G,Francesko A,Touriño S,et al.Laccase-assisted formation of bioactive chitosan/gelatin hydrogel stabilized with plant polyphenols[J].Carbohydr Polym,2013,92(2):989-996.

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