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YANG Jinhua, YIN Minmin, ZHANG Jian, WANG Lei, YE Wencai, YIN Zhiqi. Simultaneous determination of seven C21steroidal saponins in the radix of Cynanchum otophyllum Schneid.by HPLC wavelength switching method[J]. Journal of China Pharmaceutical University, 2014, 45(2): 200-204. DOI: 10.11665/j.issn.1000-5048.20140212
Citation: YANG Jinhua, YIN Minmin, ZHANG Jian, WANG Lei, YE Wencai, YIN Zhiqi. Simultaneous determination of seven C21steroidal saponins in the radix of Cynanchum otophyllum Schneid.by HPLC wavelength switching method[J]. Journal of China Pharmaceutical University, 2014, 45(2): 200-204. DOI: 10.11665/j.issn.1000-5048.20140212
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Simultaneous determination of seven C21steroidal saponins in the radix of Cynanchum otophyllum Schneid.by HPLC wavelength switching method

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  • An HPLC method was established to evaluate the quality of Cynanchum otophyllum with a simultaneous determination of seven C21 steroidal saponins. The method was carried out on an Alltima C18 column(250 mm×4. 6 mm, 5 μm)with a mobile phase consisting of acetonitrile-water at the flow rate of 1. 0 mL/min. The detection wavelength was 259 nm(0-27 min), 223 nm(27-72 min)and column temperature was 35 ℃. The seven compounds were completely separated with good linearity. The average recoveries varied from 98. 5% to 103. 9%. These results indicated that the method was convenient, accurate and rapid for the quality control of Radix of Cynanchum otophyllum in 7 commercial samples.
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