• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
YANG Jinhua, YIN Minmin, ZHANG Jian, WANG Lei, YE Wencai, YIN Zhiqi. Simultaneous determination of seven C21steroidal saponins in the radix of Cynanchum otophyllum Schneid.by HPLC wavelength switching method[J]. Journal of China Pharmaceutical University, 2014, 45(2): 200-204. DOI: 10.11665/j.issn.1000-5048.20140212
Citation: YANG Jinhua, YIN Minmin, ZHANG Jian, WANG Lei, YE Wencai, YIN Zhiqi. Simultaneous determination of seven C21steroidal saponins in the radix of Cynanchum otophyllum Schneid.by HPLC wavelength switching method[J]. Journal of China Pharmaceutical University, 2014, 45(2): 200-204. DOI: 10.11665/j.issn.1000-5048.20140212
shu

Simultaneous determination of seven C21steroidal saponins in the radix of Cynanchum otophyllum Schneid.by HPLC wavelength switching method

More Information
    Graphical Abstract
    • Abstract
  • An HPLC method was established to evaluate the quality of Cynanchum otophyllum with a simultaneous determination of seven C21 steroidal saponins. The method was carried out on an Alltima C18 column(250 mm×4. 6 mm, 5 μm)with a mobile phase consisting of acetonitrile-water at the flow rate of 1. 0 mL/min. The detection wavelength was 259 nm(0-27 min), 223 nm(27-72 min)and column temperature was 35 ℃. The seven compounds were completely separated with good linearity. The average recoveries varied from 98. 5% to 103. 9%. These results indicated that the method was convenient, accurate and rapid for the quality control of Radix of Cynanchum otophyllum in 7 commercial samples.
    • FullText(HTML)
    • References (16)
  • [1]
    Jiang Y,Li BT.Flora of China(中国植物志)[M].Beijing:Science Press,1977:377.
    [2]
    Sun YN,Yan JL.Clinical application of Cynanchum otophyllum Schneid.as a national medicine[J].Chin J Ethnomed Ethnopharm(中国民族民间医药杂志),1999,(5):309.
    [3]
    Zhao XG,Tang LJ.Observation on the treatment of 20 cases of intractable epilepsy by Cynanchum otophyllum Schneid.[J].Shaanxi Med J(陕西新医药),1985,14(8):22-23.
    [4]
    Xue SX,Yuan WC,Yang CM.Clinical analysis on the treatment of 20 cases of intractable epilepsy of children by Cynanchum otophyllum Schneid.[J].J Applied Clin Pediat(实用儿科临床杂志),1991,6(4):1931.
    [5]
    Mu QZ,Shen YM,Zhou XL,et al.Extraction of novel otophyllosides C-G from Cynanchum otophyllum Schneid.for treatment of hepatitis and Meniere′s syndrome:CN,1150951[P].1997-07-04[2013-10-20] .
    [6]
    Mu QZ,Shen YM,Zhou XL,et al.Two new antiepilepsy compounds otophlloside A and B:CN,1148958[P].1997-05-07[2013-10-20] .
    [7]
    Peng YR,Ding YF,Wei YJ,et al.Caudatin-2,6-dideoxy-3-O-methy-β-D-cymaropyranoside induced apoptosis through caspase 3-dependent pathway in human heaptoma cell line SMMC-7721[J].Phytoth Res,2011,25(5):631-637.
    [8]
    Li YB.Application of caudatin-3-O-β-D-cymarin in preparation of medicine for treating liver cancer:CN,101011404[P].2007-08-08[2013-10-20] .
    [9]
    Peng YR,Li YB,Liu XD,et al.Antitumor activity of C21 steroidal glycosides from Cynanchum auriculatum Royle exWight[J].Phytomedicine,2008,15:1 016-1 020.
    [10]
    Chen SY,Li LY,Chen Y,et al.Effect on lymphocyte in immunosuppressant mice of C21 steroid saponins in Cynanchum otophyllum Schneid.[J].Pharmacol Clin Chin Mater Med(中药药理与临床),1988,4(4):51-52.
    [11]
    Sun SK,Tian SH,Li XP.Mechanism of Qingyangshen glycosides(QYS)treating on liver injury in mice[J].Ningxia Med J(宁夏医学杂志),2010,32(2):120-122.
    [12]
    Ming QZ,Dong Y,Zhou XY.RP-HPLC assay for cynanchogenin in qingyangshen tablets[J].Chin J Pharm Anal(药物分析杂志),2006,26(8):1 156-1 157.
    [13]
    Li WL,He WJ,Li HQ,et al.Determination of cynanchogenin in Cynanchum otophyllum Schneid.by HPLC[J].West China J Pharm Sci(华西药学杂志),2009,24(6):663-664.
    [14]
    Mu QZ,Shen YM,Zhou XL,et al.Appication of otophylloside A and otophylloside B in pharmaceutical industry:CN,1148958[P].1997-05-07[2013-10-25] .
    [15]
    Wu J,Ji L,Wang LF,et al.RP-HPLC with UV Switch determination of paeoniflorin and pepper alkali in Zhitong tablets[J].Chin J Exp Tradit Med Formulae(中国实验方剂学杂志),2011,19(15):140-142.
    [16]
    Li WM, Zhao YR, Yang YY, et al. RP-HPLC with UV switch determination of 9 components in white peony root pieces[J].Chin J Pharm Anal(药物分析杂志),2011,31(12):2 208-2 012.
    • Related Articles

  • Related Articles

    [1]WANG Shihao, LIU Lifeng, DING Yang, LI Suxin. Research progress of pH-responsive drug delivery systems in cancer immunotherapy[J]. Journal of China Pharmaceutical University, 2024, 55(4): 522-529. DOI: 10.11665/j.issn.1000-5048.2024011902
    [2]SUN Liting, ZHANG Weiguo, TONG Yue. Research progress on targeted protein S-palmitoylation modification in T cell immunotherapy[J]. Journal of China Pharmaceutical University, 2024, 55(1): 45-52. DOI: 10.11665/j.issn.1000-5048.2023112903
    [3]CUI Zhenzhen, ZHAO Yifan, SUN Yu, Meng Jiayi, KANG Di, HU Lihong. Research progress of drugs for cancer immunotherapy based on CCL2/CCR2 signaling axis[J]. Journal of China Pharmaceutical University, 2024, 55(1): 36-44. DOI: 10.11665/j.issn.1000-5048.2023112904
    [4]YAO Zheng, LI Zihan, GAO Liming, HU Xing, CHEN Yan, PAN Wenqi, LI Qian. Advances of research on CAR-T cell immunotherapy for solid tumors[J]. Journal of China Pharmaceutical University, 2021, 52(4): 496-504. DOI: 10.11665/j.issn.1000-5048.20210413
    [5]YU Zexuan, JU Caoyun, ZHANG Can. Construction and application of gene delivery systems for primary dendritic cells[J]. Journal of China Pharmaceutical University, 2021, 52(4): 438-446. DOI: 10.11665/j.issn.1000-5048.20210406
    [6]XING Xuyang, WANG Xiaochun, HE Wei. Advances in research on tumor immunotherapy and its drug development[J]. Journal of China Pharmaceutical University, 2021, 52(1): 10-19. DOI: 10.11665/j.issn.1000-5048.20210102
    [7]JIANG Liangliang, JIANG Tao, LUO Jianhua, YAO Wenbing, TIAN Hong. A novel human immune system mice model for assessing the immunogenicity of cancer vaccines[J]. Journal of China Pharmaceutical University, 2019, 50(6): 734-742. DOI: 10.11665/j.issn.1000-5048.20190615
    [8]WANG Rui, WANG Yongmei, CAI Mingjun, KE Xuejia, WU Yue, CHONG Jun, CAO Rongyue. Pharmacological effects of anti-melanoma DC vaccine sensitized by fusion proteins of G3G6 and HST1[J]. Journal of China Pharmaceutical University, 2019, 50(2): 238-245. DOI: 10.11665/j.issn.1000-5048.20190216
    [9]ZHANG Rui, JIANG Tao, ZENG Tianzhu, YAO Wenbing, GAO Xiangdong, TIAN Hong. Advances of combined immunotherapy in tumor[J]. Journal of China Pharmaceutical University, 2018, 49(4): 383-391. DOI: 10.11665/j.issn.1000-5048.20180401
    [10]JING Liangliang, MIAO Zitao, LI Manman, YE Jia, JIN Liang, CAO Rongyue, LONG Jun. Anti-tumor effect and its mechanism of co-administration of fusion proteins hVEGF121/βhCG and mGM-CSF/βhCG[J]. Journal of China Pharmaceutical University, 2017, 48(1): 102-109. DOI: 10.11665/j.issn.1000-5048.20170116

Catalog

    Get Citation
    PDF
    XML
    Article views (1178) PDF downloads (1992) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：297244

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return