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HU Siyao, ZHOU Yu, TAO Chunlei, SONG Xin, XU Fan, WANG Yunlai. HPLC-DAD fingerprint of Danggui-Shaoyao-San and its synergy[J]. Journal of China Pharmaceutical University, 2014, 45(2): 205-209. DOI: 10.11665/j.issn.1000-5048.20140213
Citation: HU Siyao, ZHOU Yu, TAO Chunlei, SONG Xin, XU Fan, WANG Yunlai. HPLC-DAD fingerprint of Danggui-Shaoyao-San and its synergy[J]. Journal of China Pharmaceutical University, 2014, 45(2): 205-209. DOI: 10.11665/j.issn.1000-5048.20140213
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HPLC-DAD fingerprint of Danggui-Shaoyao-San and its synergy

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  • An HPLC-DAD fingerprint method was developed for the simultaneous determination of Danggui-Shaoyao-San. A Welchrom C18 column(250 mm×4. 6 mm, 5 μm)was used with a gradient mobile phase system of acetonitrile-water containing 0. 05% phosphoric acid. The detection wavelength was 230 nm; the flow rate was 1 mL/min. From the fingerprint we can conclude that in the total 21 peaks, 8 was from Angelicae sinensis, 11 from Paeonia lactiflora, 13 from the Ligustici Chuanxiong, 4 from Atractylodis macrocephalae, 2 from Poria cocos, and 3 from Rhizoma Alismatis. The validated method was applied to the quality control of Danggui-Shaoyao-San, providing a basis for the efficacy of various parts to clarify the material basis.
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    • References (8)
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