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MU Haifeng, LI Huijun, CHEN Jun, LI Ping. Simultaneous determination of three sterones in Achyranthis bidentatae Radix by RP-HPLC[J]. Journal of China Pharmaceutical University, 2014, 45(2): 210-212. DOI: 10.11665/j.issn.1000-5048.20140214
Citation: MU Haifeng, LI Huijun, CHEN Jun, LI Ping. Simultaneous determination of three sterones in Achyranthis bidentatae Radix by RP-HPLC[J]. Journal of China Pharmaceutical University, 2014, 45(2): 210-212. DOI: 10.11665/j.issn.1000-5048.20140214
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Simultaneous determination of three sterones in Achyranthis bidentatae Radix by RP-HPLC

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  • An HPLC method was established for the simultaneous determination of β-ecdysterone, R-inokosterone and S-inokosterone in the Achyranthis bidentatae Radix to compare the quality from different habitats. Three phytoecdysones were separated with a Venusil XBP C18 column by isocratic elution using 0. 1% formic acid in water and acetonitrile(84 ∶16)as the mobile phase, while the UV detector wavelength was set at 250 nm. Calibration curves of β-ecdysterone, R-inokosterone and S-inokosterone were linear over the range of 3. 07-1 470. 00, 0. 52-246. 00, and 0. 47-225. 00 μg/mL, respectively. Average recoveries were 101. 4%, 101. 1% and 101. 1%, respectively. The established method will be helpful in the quality control of Achyranthis bidentatae Radix.
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    • References (3)
  • [1]
    Chinese Pharmacopoeia Commission.Chinese Pharmacopoeia:part 1(中华人民共和国药典:一部)[S].Beijing:China Medical Science Press,2010:67.
    [2]
    Shen S,Wang Q,Li YB.Studies on chemical constituents an pharmaceutics activity of Achyranthes bidentata Bl[J].Strait Pharma J(海峡药学),2011,23(11):1-6.
    [3]
    Sun FY,Pan QH,Hong A.The Effect of promotion osteoblast proliferation and mechanism research of Achyranthes[J].J Chin Mater Med(中药材),2004,27(4):264-265.
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