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XU Jie, HU Wenhui, GU Dongfei, HANG Taijun. Identification and determination of related substances in atropine sulfate with hyphenated technique[J]. Journal of China Pharmaceutical University, 2014, 45(4): 438-443. DOI: 10.11665/j.issn.1000-5048.20140410
Citation: XU Jie, HU Wenhui, GU Dongfei, HANG Taijun. Identification and determination of related substances in atropine sulfate with hyphenated technique[J]. Journal of China Pharmaceutical University, 2014, 45(4): 438-443. DOI: 10.11665/j.issn.1000-5048.20140410
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Identification and determination of related substances in atropine sulfate with hyphenated technique

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  • A specific ion-pairing HPLC method was developed for the determination of related substances in atropine sulfate. Their structures were identified by LC-MS/MS. HPLC separation was carried out on a Thermo BDS Hypersil C18 column with linear gradient elution by a mobile phase consisting of acetonitrile and phosphate buffer solution(containing sodium heptane-sulphonate, adjusting pH to 5. 0 with phosphoric acid). The flow rate was 0. 8 mL/min and UV detection wavelength was 225 nm. Atropine sulfate and its related substances can be completely separated under the established HPLC condition. And they were characterized by hyphenated chromatographic techniques with accurate mass determination using high resolution LC-TOF/MS methods as well as product MS spectra scanning through a volatile mobile phase. Twelve related substances were detected and identified as by-products from synthesis and degradations of atropine sulfate. Six of them were related substances from EP and the other six were unknown related substances. This newly established HPLC method is effective in the separation and identification of the related substances in atropine sulfate and the results are useful for its quality control and process optimization.
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    • References (8)
  • [1]
    Cherkaoui S,Mateus L,Veuthey J,et al.Development and validation of a capillary zone electrophoresis method for the determination of atropine,homatropine and scopolamine in ophthalmic solutions[J].J Chromatogr B,1997,696(2):283-290.
    [2]
    Ceyhan T,Kartal M,Altun ML,et al.LC determination of atropine sulfate and scopolamine hydrobromide in pharmaceuticals[J].J Pharm Biomed Anal,2001,25(3):399-406.
    [3]
    Jakabová S,Vincze L,Farkas Á,et al.Determination of tropane alkaloids atropine and scopolamine by liquid chromatography-mass spectrometry in plant organs of Datura species[J].J Chromatogr A,2012,1 232:295-301.
    [4]
    European Pharmacopoeia(欧洲药典)[S].7.0(第7版).Monographs:1 429-1 430.
    [5]
    Chinese Pharmacopoeia Commission. Chinese Pharmacopoeia: part 2(中华人民共和国药典:二部)[M].Beijing:China Medical Science Press,2010:981-982.
    [6]
    Bitar Y, Holzgrabe U. Impurity profiling of atropine sulfate by microemulsion electrokinetic chromatography[J].J Pharm Biomed Anal,2007,44(3):623-633.
    [7]
    Kirchhoff C,Bitar Y,Holzgrabe U,et al.Analysis of atropine,its degradation products and related substances of natural origin by means of reversed-phase high-performance liquid chromatography[J].J Chromatogr A,2004,1 046(1):115-120.
    [8]
    The First Pharmaceutical Factory of Hangzhou.Synthesis process of atropine sulfate[J].Chin J Pharm(中国医药工业杂志),1972,8:15-17.
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