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CHEN Lei, YANG Ping, JI Yibing. Chiral separation of esomeprazole and its enantiomer by affinity capillary electrophoresis[J]. Journal of China Pharmaceutical University, 2014, 45(4): 444-449. DOI: 10.11665/j.issn.1000-5048.20140411
Citation: CHEN Lei, YANG Ping, JI Yibing. Chiral separation of esomeprazole and its enantiomer by affinity capillary electrophoresis[J]. Journal of China Pharmaceutical University, 2014, 45(4): 444-449. DOI: 10.11665/j.issn.1000-5048.20140411
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Chiral separation of esomeprazole and its enantiomer by affinity capillary electrophoresis

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  • An affinity capillary electrophoresis(ACE)method with bovine serum albumin(BSA)as chiral selector was developed to test the enantiomeric impurity in esomeprazole. The mechanism of enantioseparation of esomeprazole and its enantiomer via BSA was investigated by fluorescence and circular dichroism spectrum for thermodynamic parameters, enthalpy entropy-driven process and the conformational changes of BSA. The optimal separation condition obtained was a running buffer consisting of 20 mmol/L sodium dihydrogen phosphate and disodium hydrogen phosphate with 250 μmol/L BSA and 7% n-propanol at pH 8. 0, a positive voltage of 30 kV, separation temperature at 25 ℃ and detection wavelength at 302 nm. The resolution of esomeprazole and its enantiomer was more than 1. 8. The LOD and LOQ were 0. 8 μg/mL and 2. 0 μg/mL respectively, for the enantiomer of esomeprazole, and the linear range was 2-20 μg/mL. The average recovery of R-omeprazole was 100. 4%(RSD< 2. 0%). This method could be applied to test the enantiomeric impurity of esomeprazole drug substances.
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  • [1]
    Zhang SX.The research process of esomeprazol[J].Qinghai Med J(青海医药杂志),2012,42(3):85-87.
    [2]
    Yang L, Yan BX. Pharmacokinetics of esomeprazol[J]. Chin New Drugs J(中国新药杂志),2004,13(5):398-400.
    [3]
    Shi SJ,Klotz U.Proton pump inhibitors:an update of their clinical use and pharmacokinetics[J].Eur J Clin Pharmacol,2008,64(10):935-951.
    [4]
    Wang F.Comparation of the effect of esomeprazol and omeprazol on gastric ulcer[J].Chin Pract Med(中国实用医药),2013,8(28):190-191.
    [5]
    Ma Z,Zhang LJ,Lin LN,et al.Enantioseparation of rabeprazole and omeprazole by nonaqueous capillary electrophoresis with an ephedrine-based ionic liquid as the chiral selector[J].Biomed Chromatogr,2010,24(12):1 332-1 337.
    [6]
    Olsson J,Stegander F,Marlin N,et al.Enantiomeric separation of omeprazole and its metabolite 5-hydroxyomeprazole using non-aqueous capillary electrophoresis[J].J Chromatogr A,2006,1 129(2):291-295.
    [7]
    Olsson J,Marlin ND,Blomberg LG.Enantiomeric separation of omeperazole enantiomers by aqueous CE using UV and MS detection[J].Chromatogr.2007,66(5/6):421-425.
    [8]
    Berzas Nevado JJ,Castaneda Penalvo G,Rodriguez Dorado RM.Method development and validation for the separation and determination of omeprazole enantiomers in pharmaceutical preparations by capillary electrophoresis[J].Anal Chim Acta,2005,533(2):127-133.
    [9]
    OlssonJ,Blomberg LG.Enantioseparation of omeprazole and its metabolite 5-hydroxy omeprazole using open tubular capillary electrochromatography with immobilized avidin as chiral selector[J].J Chromatogr B,2008,875(1):329-332.
    [10]
    Yang F,Du YX,Chen B,et al.Enantiomeric separation of nefopam hydrochloride by affinity electrokinetic chromatography using chondroitin sulfate A as chiral selector and its chiral recognition mechanism[J].Chromatogr,2010,72(5/6):489-493.
    [11]
    Gotti R,Parma B,Spelta F,et al.Affinity capillary electrophoresis in binding study of antithrombin to heparin from different sources[J].Talanta,2013,105(12):366-371.
    [12]
    Ding R,Ji H,Lu W,et al.Chiral separation and limit test of clopidogrel bisulfate isomers[J].J China Pharm Univ(中国药科大学学报),2012,43(3):241-246.
    [13]
    Yu JH,Li B,Dai P,et al.Molecular simulation of the interaction between novel type rhodanine derivative probe and bovine serum albumin[J].Spectrochim Acta Part A,2009,74(1):277-281.
    [14]
    Ross PD,Subramanian S.Thermodynamics of protein association reactions:forces contributing to stability[J].J Biochem,1981,20(11):3 096-3 102.
    [15]
    Li DJ,Zhu M,Xu C,et al.Characterization of the baicalein-bovine serum albumin complex without or with Cu2+or Fe3+ by spectroscopic approaches[J].Eur J Med Chem,2011,46(2):588-599.
    [16]
    Varlan A, Ionescu S, Hillebrand M. Study of the interaction between ofloxacin and human serum albumin by spectroscopic methods[J].J Lumin,2011,26(6):710-715.
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