• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
WANG Li, LI Mingdan, LU Yi, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Mechanism of apoptosis induced by peptide-rich fraction from the root of Aster tataricus in liver L-02 cells[J]. Journal of China Pharmaceutical University, 2014, 45(4): 469-474. DOI: 10.11665/j.issn.1000-5048.20140415
Citation: WANG Li, LI Mingdan, LU Yi, ZHANG Chaofeng, XU Xianghong, ZHANG Mian. Mechanism of apoptosis induced by peptide-rich fraction from the root of Aster tataricus in liver L-02 cells[J]. Journal of China Pharmaceutical University, 2014, 45(4): 469-474. DOI: 10.11665/j.issn.1000-5048.20140415
shu

Mechanism of apoptosis induced by peptide-rich fraction from the root of Aster tataricus in liver L-02 cells

More Information
    Graphical Abstract
    • Abstract
  • To evaluate the hepatotoxicity and its mechanism of the peptide-rich fraction(Fr-2)of the root of Aster tataricus in human liver L-02 cells. Cell viability was assessed by MTT assay and the contents of LDH, ROS, GSH, cytochrome C and Caspase-9/3 were assessed by commercially available kits. Cell apoptosis and mitochondrial membrane potential were measured by flow cytometry. The protein expressions of p-JNK, Bax, Bcl-2 were analysed by Western blot assay. The results demonstrated that the Fr-2 fraction induced apoptosis in a concentration- and time-dependent manner and provoked oxidative stress-associated inflammation in L-02 cells. It was characterized by loss of mitochondrial membrane potential and release of cytochrome c from mitochondria. The enhanced Bax/Bcl-2 ratio and the activated Caspase-9/3 indicated that the Fr-2 fraction induces apoptosis via mitochondria-dependent pathway in liver L-02 cells.
    • FullText(HTML)
    • References (14)
  • [1]
    China Pharmacopoeia Committee.Chinese Pharmacopoeia[M].Vol.I.Beijing:The Medicine Science and Technology Press of China(中国医药科技出版社),2010:322.
    [2]
    Zhang JW,Dou CG,Zhang M,et al.Toxicity of Radix Asteris,Flos Farfarae and their combination[J].Chin J Clin Pharmacol Ther(中国临床药理学与治疗学),2007,12(4):405-411.
    [3]
    Shao J,Jin J,Zhang M,et al.Toxic part of Radix Asteris and antagonizing part of Flos Farfarae in pair-drug of FF and RA[J].Lishizhen Med Mater Med Res(时珍国医国药),2009,20(6):1 308-1 310.
    [4]
    Wang L,Zhang M,Jin J,et al.Toxic fraction of Radix Asteris and its acute hepatotoxicity to mice[J].Lishizhen Med Mater Med Res(时珍国医国药),2010,21(10):2 526-2 528.
    [5]
    Liu XD,Cao P,Zhang C,et al.Screening and analyzing potential hepatotoxic compounds in the ethanol extract of Asteris Radix by HPLC/DAD/ESI-MSn technique[J].J Pharm Biomed Anal,2012,67-68,51-62.doi: 10.1016/j.jpba.2012.04.034.
    [6]
    Tyurin VA,Tyurina YY,Feng W,et al.Mass-spectrometric characterization of phospholipids and their primary peroxidation products in rat cortical neurons during staurosporine-induced apoptosis[J].J Neurochem,2008,107(6):1 614-1 633.
    [7]
    Cao PP.Apoptosis of L-02 cells induced by the toxic components of Asteris tataricus[D].Nanjing:China Pharmaceutical University,2012.
    [8]
    Malhi H,Guicciardi ME,Gores GJ.Hepatocyte death:a clear and present danger[J].Physiol Rev,2010,90(3):1 165-1 194.
    [9]
    Pessayre D,Fromenty B,Berson A,et al.Central role of mitochondria in drug-induced liver injury[J].Drug Metab Rev,2012,44(1):34-87.
    [10]
    Jaeschke H,Williams CD,Mcgill MR,et al.Models of drug-induced liver injury for evaluation of phytotherapeutics and other natural products[J].Food Chem Toxicol,2013,55:279-289.
    [11]
    Kaplowitz N.Mechanisms of liver cell injury[J].J Hepatol,2000,3(suppl.1):39-47.
    [12]
    Danial NN,Korsmeyer SJ.Cell death:critical control points[J].Cell,2004,116(2):205-219.
    [13]
    Cozzolino R,Palladino P,Rossi F,et al.Antineoplastic cyclic astin analogues kill tumour cells via caspase-mediated induction of apoptosis[J].Carcinogenesis,2005,26(4):733-739.
    [14]
    Shen Y,Luo Q,Xu H M,et al.Mitochondria-dependent apoptosis of activated T lymphocytes induced by astin C,a plant cyclopeptide,for preventing murine experimental colitis[J].Biochem Pharmacol,2011,82(3):260-268.
    • Related Articles

  • Related Articles

    [1]ZHANG Huili, LI Ke, ZHAO Hui, HU Guoqiang, HUANG Wenlong. Synthesis and antitumor activity of fluoroquinolone C-3 s-triazole Schiff-base carboxylic acid derivatives from pefloxacin(X)[J]. Journal of China Pharmaceutical University, 2017, 48(2): 167-171. DOI: 10.11665/j.issn.1000-5048.20170206
    [2]YAN Qiang, WU Shumin, NI Lili, XIE Yusuo, GAO Liuzhou, HUANG Wenlong, LIU Yingjie, HU Guoqiang. Synthesis and antitumor activity of C-3 thiazolo ［3, 2-b］［1, 2, 4］triazole-substituted pefloxacin derivatives[J]. Journal of China Pharmaceutical University, 2015, 46(5): 548-551. DOI: 10.11665/j.issn.1000-5048.20150505
    [3]QIAO Yixue, MOU Yi, HUANG Zhangjian, AI Yong, KANG Fenghua, LAI Yisheng, ZHANG Yihua. Synthesis and antitumor activities of novel CDDO-Me analogues[J]. Journal of China Pharmaceutical University, 2015, 46(3): 289-293. DOI: 10.11665/j.issn.1000-5048.20150305
    [4]CHEN Shumin, HUANG Wenlong, HU Guoqiang. Synthesis and antitumor activities of fluoroquinolone C-3 isosteres(VI):s-triazole-acylhydrazone methylsulfide derivatives[J]. Journal of China Pharmaceutical University, 2014, 45(5): 511-516. DOI: 10.11665/j.issn.1000-5048.20140501
    [5]SUN Yusheng, XU Qiuju, HOU Lili, YUE Xibo, WU Zhaofeng, HUANG Wenlong, XIE Songqiang, HU Guoqiang. Synthesis and antitumor activities of fluoroquinolone C-3 isosteres(IV):s-triazole-oxadiazole methylsulfide Mannich-base derivatives[J]. Journal of China Pharmaceutical University, 2014, 45(1): 39-42. DOI: 10.11665/j.issn.1000-5048.20140106
    [6]HU Kun, ZHOU Anfei, JIANG Hefei, XU Yuanyuan, HUANG Qianhui, YANG Jie, CHEN Xin, REN Jie. Synthesis and antitumor activities of podophyllotoxin derivatives[J]. Journal of China Pharmaceutical University, 2014, 45(1): 33-38. DOI: 10.11665/j.issn.1000-5048.20140105
    [7]XU Qiuju, HOU Lili, WU Zhaofeng, YUE Xibo, HU Guoqiang, HUANG Wenlong. Synthesis and antitumor activity of fluoroquinolone C-3 isostere Ⅲ:s-triazole oxadiazole methylsulfide derivatives from pefloxacin[J]. Journal of China Pharmaceutical University, 2013, 44(6): 511-514. DOI: 10.11665/j.issn.1000-5048.20130604
    [9]Synthesis of the Derivatives of 2-Acetylbenzimidazolyl Thiosemicarbazone[J]. Journal of China Pharmaceutical University, 1990, (5): 295-297.
    [10]SYNTHESIS OF 2-ACETYLTHIOPHENE THIOSEMICARBAZONE DERIVATIVES[J]. Journal of China Pharmaceutical University, 1990, (1): 6-10.

Catalog

    Get Citation
    PDF
    XML
    Article views (991) PDF downloads (1382) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：303101

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return