• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
ZHAO Zekai, WANG Lu, XUE Jingwei, ZHANG Can. Development of reduction response probes[J]. Journal of China Pharmaceutical University, 2014, 45(5): 535-539. DOI: 10.11665/j.issn.1000-5048.20140505
Citation: ZHAO Zekai, WANG Lu, XUE Jingwei, ZHANG Can. Development of reduction response probes[J]. Journal of China Pharmaceutical University, 2014, 45(5): 535-539. DOI: 10.11665/j.issn.1000-5048.20140505
shu

Development of reduction response probes

More Information
    Graphical Abstract
    • Abstract
  • Based on fluorescence resonance energy transfer(FRET)principle and the reductive environment in the cytoplasm, this study attempted to characterize the stability of the carrier in cells. A new probe conjugation with FRET and reduction responsibility simultaneously, (3-COOH-7-OH-coumarin)-(sulfur-sulfur)-(fluorescein isothiocyanate)(CHC-SS-FITC), was first synthesized. With liposome being used as model carrier, (CHC-SS-FITC)-liposome was prepared and the FRET effect and reduction responsibility of CHC-SS-FITC were assessed. The stability of drug-loading liposome in macrophages was investigated by laser scan confocal microscopy. Results showed that the reduction response FRET probes(CHC-SS-FITC)could be used to characterize the stability of nano-carrier in cytoplasm, due to the high sensitivity and strong counter-interference of the carrier.
    • FullText(HTML)
    • References (10)
  • [1]
    Dennis AM,Rhee WJ,Sotto D,et al.Quantum dot-fluorescent protein FRET probes for sensing intracellular pH[J].ACS Nano,2012,6(4):2 917-2 924.
    [2]
    Alabi CA,Love KT,Sahay G,et al.FRET-labeled siRNA probes for tracking assembly and disassembly of siRNA nanocomplexes[J].ACS Nano,2012,6(7):6 133-6 141.
    [3]
    Bunschoten A,Buckle T,Kuil J,et al.Targeted non-covalent self-assembled nanoparticles based on human serum albumin[J].Biomaterials,2012,33(3):867-875.
    [4]
    Chen KJ,Chiu YL,Chen YM,et al.Intracellularly monitoring/imaging the release of doxorubicin from pH-responsive nanoparticles using Forster resonance energy transfer[J].Biomaterials,2011,32(10):2 586-2 592.
    [5]
    Liu HY,Wu SM,Cheng F,et al.Fluorescence resonance energy transfer based on quantum dots[J].Chin J Anal Chem(分析化学),2010,38(7):1 036-1 039.
    [6]
    Wang YQ,Ye C,Wang XM,et al.Application trends of fluorescent quantum dots in the research of nanomedicines [J]. J China Pharm Univ(中国药科大学学报),2009,40(1):8-15.
    [7]
    Lai J,Shah BP,Garfunkel E,et al.Versatile fluorescence resonance energy transfer-based mesoporous silica nanoparticles for real-time monitoring of drug release[J].ACS Nano,2013,7(3):2 741-2 750.
    [8]
    Lushchak VI.Glutathione homeostasis and functions:potential targets for medical interventions[J].J Amino Acids,2012,2 012:736 837.
    [9]
    Mo R,Sun Q,Xue J,et al.Multistage pH-responsive liposomes for mitochondrial-targeted anticancer drug delivery[J].Adv Mater,2012,24(27):3 659-3 665.
    [10]
    Ye SS,Zeng YY,Yin LL.Effects of salidroside on proliferation,apoptosis,phagocytosis,ROS and NO production of murine peritoneal macrophages in vitro [J].Chin J Cell Mol Immunol(细胞与分子免疫学杂志),2011,27(3):237-241.
    • Related Articles

  • Related Articles

    [1]ZHAO Yan-rong, DING Li, RAO Ya-kun, TANG Xiao-wen, DING Jun-hong. Stability and degradation products of otilonium bromide in different media[J]. Journal of China Pharmaceutical University, 2010, 41(6): 548-554.
    [2]YUAN Xue, ZOU Chang, JIANG Li-ping, ZHANG Yun, CHEN Ya-li. In vitro stability of Endostar[J]. Journal of China Pharmaceutical University, 2009, 40(4): 359-363.
    [3]Content Determination and Stability Research on Diclofenac-Zn[J]. Journal of China Pharmaceutical University, 2002, (3): 92-93.
    [4]Application of Thermogravimetry to the Study of Thermal Decomposition Kinetics of Cefalexin and its Stability[J]. Journal of China Pharmaceutical University, 2000, (2): 16-20.
    [5]Appliacation of Differential Scanning Carolimetry to the Study of the Relationship Between Yohimbine Hydrochloride''s Stability and its Excipients[J]. Journal of China Pharmaceutical University, 1998, (6): 427-428.
    [6]Studies on Stability of Lomefloxacin Hydrochloride Injection[J]. Journal of China Pharmaceutical University, 1996, (6): 23-26.
    [7]Studies on Oxyfedrine Hydrochloride Injection and Its Stability[J]. Journal of China Pharmaceutical University, 1995, (1): 17-19.
    [8]Study on Stabilities of Heparin Modified Human Erythrocyte Superoxide Dismutase[J]. Journal of China Pharmaceutical University, 1994, (6): 373-375.
    [9]DISCUSSION OF STABILITY OF THE YIN-YANG EQUATION[J]. Journal of China Pharmaceutical University, 1987, (1): 40-44.
    [10]Ping Qineng, Zheng Liangyuan, Liu Guojie, Tu Xide. THE STABILITY OF TETRACYCLINE IN SUSPENSIONS[J]. Journal of China Pharmaceutical University, 1982, (3): 43-49.

Catalog

    Get Citation
    PDF
    XML
    Article views (1408) PDF downloads (1936) Cited by()
    Turn off MathJax
    Article Contents
    Abstract
    References
    Related Articles

    Copyright © Journal of China Pharmaceutical University苏ICP备12050101号-2

    Address：24 Tongjia Lane, Nanjing City, Jiangsu Province （210009）Tel: 025-83271566E-mail: xuebao@cpu.edu.cn

    Supported by: Beijing Renhe Information Technology Co., Ltd.  Baidu Analytics

    Total visits：319008

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return