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ZHENG Ying, LIU Wei, ZHANG Zhenzhen, GAO Xiangdong, YAO Wenbing. Purification of an acidic polysaccharide from Sarcandra glabra and its inhibitory effect on α-glycosidase activity[J]. Journal of China Pharmaceutical University, 2014, 45(5): 576-579. DOI: 10.11665/j.issn.1000-5048.20140513
Citation: ZHENG Ying, LIU Wei, ZHANG Zhenzhen, GAO Xiangdong, YAO Wenbing. Purification of an acidic polysaccharide from Sarcandra glabra and its inhibitory effect on α-glycosidase activity[J]. Journal of China Pharmaceutical University, 2014, 45(5): 576-579. DOI: 10.11665/j.issn.1000-5048.20140513
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Purification of an acidic polysaccharide from Sarcandra glabra and its inhibitory effect on α-glycosidase activity

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  • Through the preparation of the crude polysaccharide(SGP-C)from Sarcandra glabra(Thunb)Nakai, the polysaccharide of SGP-O was obtained with optimal purification conditions(pH 9, 5% H2O2, 50 °C, decolored for 3. 5 h). Then, high-quality polysaccharide(SGP-2)was purified by Sephacryl S-400 molecular sieve with the yield of 0. 35%. In vitro α-glucosidase inhibition assay indicated that SGP-2 had an IC50 of(87. 06±11. 76)μg/mL, only a quarter of that of acarbose(338. 9±46. 86 μg/mL), which indicated that SGP-2 had strong α-glucosidase enzyme inhibitory activity. Preliminary dynamic studies showed that SGP-2 inhibited α-glucosidase in a reversible and mixed-type mode.
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