Research of<i> genD</i>1 in gentamicin biosynthesis gene cluster

CHEN Zhouqin; LIN Qiang; HU Yulong; HONG Wenrong

doi:10.11665/j.issn.1000-5048.20140619

Journal of China Pharmaceutical University > 2014 > 45(6): 720-725. > DOI: 10.11665/j.issn.1000-5048.20140619

CHEN Zhouqin, LIN Qiang, HU Yulong, HONG Wenrong. Research of genD1 in gentamicin biosynthesis gene cluster[J]. Journal of China Pharmaceutical University, 2014, 45(6): 720-725. DOI: 10.11665/j.issn.1000-5048.20140619

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Research of genD1 in gentamicin biosynthesis gene cluster

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Abstract

In order to verify the function of genD1, a recombinant plasmid pGD14 was constructed for blocking-up genD1. pGD14 was introduced into Micromonospora purpurea GK1101(ΔgenK))by conjugation. A desired mutant strain GD1989 was obtained by PCR analysis. Flask shaking experiments and MS analysis showed that the mutant strain GD1989 only produced gentamicin A instead of gentamicin C complex, and the product of GD1989 feeding on gentamicin X2 contained gentamicin C complex. It was speculated that genD1 gene might be responsible for methylation at C-4″ of garosamine.
- gentamicin,
- methylation,
- biosynthesis,
- conjugation,
- genD1,
- Micromonospora purpurea

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