Preparation of mGM-CSF/βhCG fusion protein and the effect of its sensitized DC vaccine on RM-1 prostate tumor in mice

CAO Rongyue; CHANG Na; LI Manman; WANG Yunkang; WU Di; SHI Baoying; YUAN Yuting; LONG Jun

doi:10.11665/j.issn.1000-5048.20150117

Journal of China Pharmaceutical University > 2015 > 46(1): 111-116. > DOI: 10.11665/j.issn.1000-5048.20150117

CAO Rongyue, CHANG Na, LI Manman, WANG Yunkang, WU Di, SHI Baoying, YUAN Yuting, LONG Jun. Preparation of mGM-CSF/βhCG fusion protein and the effect of its sensitized DC vaccine on RM-1 prostate tumor in mice[J]. Journal of China Pharmaceutical University, 2015, 46(1): 111-116. DOI: 10.11665/j.issn.1000-5048.20150117

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Preparation of mGM-CSF/βhCG fusion protein and the effect of its sensitized DC vaccine on RM-1 prostate tumor in mice

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Abstract

An expression vector pET-28a-mGM-CSF-X10-βhCGCTP37 plasmid containing the βhCG and mGM-CSF gene was designed and constructed. The fusion protein was induced by lactose and purified by ammonium sulfate precipitation and DEAE-cellulose anion exchange column. Then dendritic cells(DC)in C57BL/6J mice were extracted and sensitized by the fusion protein to obtain DC vaccine. The DC vaccine was inoculated to C57BL of / 6J mice with prostate cancer RM-1. The results indicated that the anti-tumor effects of DC group and DC combined with paclitaxel(DP)group were superior to that of paclitaxel(Pac)group(P< 0. 01), and the anti-tumor effect of DP group was better than that of DC group. Thus, the constructed DC vaccine can inhibit the growth of prostate cancer, and have synergistic anti-tumor when used with paclitaxel.
- βhCG,
- mGM-CSF,
- antitumor,
- prostate cancer,
- fusion protein,
- immunotherapy,
- dendritic cells

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Preparation of mGM-CSF/βhCG fusion protein and the effect of its sensitized DC vaccine on RM-1 prostate tumor in mice

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