• 中国精品科技期刊
  • 中国高校百佳科技期刊
  • 中国中文核心期刊
  • 中国科学引文数据库核心期刊
Advanced Search
WANG Yizhou, FANG Kai, WANG Hui, DOU Jie, ZHOU Changlin. Synergistic inhibition of Lactobacillus acidophilus and Clostridium butyricum against Shigella flexneri in vitro[J]. Journal of China Pharmaceutical University, 2015, 46(1): 117-122. DOI: 10.11665/j.issn.1000-5048.20150118
Citation: WANG Yizhou, FANG Kai, WANG Hui, DOU Jie, ZHOU Changlin. Synergistic inhibition of Lactobacillus acidophilus and Clostridium butyricum against Shigella flexneri in vitro[J]. Journal of China Pharmaceutical University, 2015, 46(1): 117-122. DOI: 10.11665/j.issn.1000-5048.20150118

Synergistic inhibition of Lactobacillus acidophilus and Clostridium butyricum against Shigella flexneri in vitro

More Information
  • An agar diffusion method was used to investigate the antimicrobial activities of fermentation broth of Lactobacillus acidophilus(L. acidophilus), and Clostridium butyricum(C. butyricum)against Shigella flexneri(S. flexneri)infection in vitro. It was found that cell-free culture supernatants(CFCSs)of L. acidophilus and C. butyricum possessed remarkable synergistic anti-S. flexneri activity in vitro and the antimicrobial activity of the mixed culture was 17. 2% and 22. 4% greater, than single strains alone, respectively. Meanwhile, there was a symbiotic relationship between L. acidophilus and C. butyricum. The result showed that the biomass accumulation of the mixed culture reached 4. 27 g/L(DCW)and increased by 6. 0% and 30. 6% compared to the L. acidophilus and C. butyricum, respectively. Moreover, L. acidophilus and C. butyricum clearly inhibited S. flexneri adhesion to Caco-2 cells by 75. 8% and 81. 2%, and the combination of these two probiotic strains demonstrated the highest inhibition rate, reaching 84. 2%, while the viability of Caco-2 cells treated with L. acidophilus, C. butyricum or their combination was increased by 11. 5%, 12. 5% and 22. 9%, respectively. The synergistic effect of L. acidophilus and C. butyricum provided better protection against Shigella flexneri infection, which represents a promising alternative therapy for shigellosis.
  • [1]
    Boirivant M,Strober W.The mechanism of action of probiotics[J].Curr Opin Gastroenterol,2007, 23(6):679-692.
    [2]
    Jeon SG,Kayama H,Ueda Y, et al.Probiotic Bifidobacterium breve induces IL-10-producing Tr1 cells in the colon[J].PLoS Pathog,2012, 8(5):e1002714.
    [3]
    Tejero-Sariñena S,Barlow J,Costabile A, et al.In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens:Evidence for the effects of organic acids[J].Anaerobe,2012, 18(5):530-538.
    [4]
    Gao Q,Qi L,Wu T, et al.An important role of interleukin-10 in counteracting excessive immune response in HT-29 cells exposed to Clostridium butyricum[J].BMC Microbiol,2012, 12:100.
    [5]
    Cristiano P,Rubina S,Giorgos B, et al.Probiotics promote gut health through stimulation of epithelial innate immunity[J].PNAS,2010, 107(1):454-459.
    [6]
    Kwon HK,Lee CG,So JS, et al.Generation of regulatory dendritic cells and CD4+ Foxp3+ T cells by probiotics administration suppresses immune disorders[J].PNAS,2010, 107(5):2159-2164.
    [7]
    Moorthy G,Murali MR,Devaraj SN.Lactobacilli facilitate maintenance of intestinal membrane integrity during Shigella dysenteriae 1 infection in rats[J].Nutrition,2005, 25(3):350-358.
    [8]
    Shim DH,Suzuki T,Chang SY, et al.New animal model of shigellosis in the guinea pig:its usefulness for protective efficacy studies[J].J Immunol,2007, 178(4):2476-2482.
    [9]
    Njuguna HN,Cosmas L,Williamson J, et al.Use of population-based surveillance to define the high incidence of Shigellosis in an urban slumin nairobi,Kenya[J].PLoS One,2013, 8(3):e58437.
    [10]
    Wu L,Wang G,Gao F.Study on the mechanism of anti-Shigella flexneri infection by Bifidobacteria[J].J Dali Univ(大理学院学报),2010, 9(6):16-18.
    [11]
    Ram PK,Crump JA,Gupta SK, et al.Analysis of data gaps pertaining to Shigella infections in low and medium human development index countries,1984-2005[J].Epidemiol Infect,2008, 136(5):577-603.
    [12]
    Chang Z,Lu S,Chen L, et al.Causative species and serotypes of shigellosis in mainland China:systematic review and meta-analysis[J].PLoS One,2012, 7(12):e52515.
    [13]
    Mandal J,V G,Emelda J, et al.The recent trends of shigellosis:a JIPMER perspective[J].J Clin Diagn Res,2012, 6(9):1474-1477.
    [14]
    Johnston BC,Ma SS,Goldenberg JZ, et al.Probiotics for the prevention of Clostridium difficile-associated diarrhea:a systematic review and meta-analysis[J].Ann Intern Med,2012, 157(12):878-888.
    [15]
    Huang J,Han M,Yu X.Characteristics and culture conditions of a forage Clostridium butyricum(一株饲用酪酸菌特性及培养条件的研究)[J].Feed Ind(饲料工业),2004, 25(2):22-25.
    [16]
    Zhang Y,Zhang L,Du M, et al.Antimicrobial activity against Shigella sonnei and probiotic properties of wild Lactobacilli from fermented food[J].Microbiol Res,2011, 167(1):27-31.
    [17]
    Parsot C, Sansonetti PJ. Invasion and pathogenesis of Shigella infections[J].Curr Top Microbiol Immunol,1996, 209:25-42.
    [18]
    Zhang CY.Screening and reaction mechanism of probiotic bacteria inhibition of Shigella sonnei(抑制宋内志贺益生菌株的筛选及作用机制研究)[D].Heilongjiang:Harbin Institute of Technology,2011.
    [19]
    Chen XN.Study on the interaction between Bifidobactrium longum NCC2705 and human intestinal epithelial cell line Caco-2(长双歧杆菌NCC2705与宿主细胞Caco-2相互作用的研究)[D].Jilin:Jilin University,2010.
    [20]
    Porter CK,Choi D,Cash B, et al.Pathogen-specific risk of chronic gastrointestinal disorders following bacterial causes of foodborne illness[J].BMC Gastroenterol,2013,13:46.
  • Related Articles

    [1]TU Penghuang, YAO Zhiren, JIN Meiling, NING Guanyu, HUANG Yaping, PAN Ke, YIN Zhiqi. Study of glycosides from Piper sintenense Hatusima[J]. Journal of China Pharmaceutical University, 2024, 55(2): 202-208. DOI: 10.11665/j.issn.1000-5048.2023122704
    [2]CAI Caihong, ZHENG Hao, GAI Cuijuan, DAI Haofu, MEI Wenli, CHEN Huiqin. Secondary metabolites of the endophytic fungus Aspergillus sp.Dq-25 from barnacle[J]. Journal of China Pharmaceutical University, 2023, 54(1): 62-67. DOI: 10.11665/j.issn.1000-5048.20220531001
    [3]FANG Siyang, ZHANG Xinggui, YE Yu, WANG Keqing, YAO Zhiren, HUANG Yaping, ZHANG Jian, QIN Yanhua, YIN Zhiqi. A new ester glycoside compound from Cyclocarya paliurus[J]. Journal of China Pharmaceutical University, 2023, 54(1): 56-61. DOI: 10.11665/j.issn.1000-5048.20221209002
    [4]XIE Jing, FAN Chunlin, XU Jie, ZHANG Jian, YE Wencai, ZHANG Xiaoqi. Alkaloids of Ervatamia pandacaqui[J]. Journal of China Pharmaceutical University, 2021, 52(3): 287-292. DOI: 10.11665/j.issn.1000-5048.20210304
    [5]ZHANG Yilun, ZHAO Xinyi, DONG Shubo, DONG Chao, SHEN Wenbin, DONG Haijuan. Isolation and identification of unknown impurities of alogliptin benzoate[J]. Journal of China Pharmaceutical University, 2020, 51(4): 488-493. DOI: 10.11665/j.issn.1000-5048.20200415
    [6]TAO Junjie, YANG Jie, WEN Xiaodong. Studies on the chemical constituents in the aerial parts of Paris polyphylla var. Yunnanensis[J]. Journal of China Pharmaceutical University, 2020, 51(4): 454-460. DOI: 10.11665/j.issn.1000-5048.20200410
    [7]HUANG Qilong, ZHANG Wanjin, LI Yan, CHEN Juan, ZHOU Baoping, ZOU Xiaohan, ZHANG Chunlei, CAO Zhengyu. Alkaloid constituents from Corydalis decumbens[J]. Journal of China Pharmaceutical University, 2017, 48(5): 563-567. DOI: 10.11665/j.issn.1000-5048.20170509
    [8]WANG Jingyi, DONG Yangyang, QI Chulu, ZHANG Jiayao, ZHANG Minjie, WANG Hao. Chemical constituents of the stem bark of Bombax ceiba L.[J]. Journal of China Pharmaceutical University, 2016, 47(5): 570-574. DOI: 10.11665/j.issn.1000-5048.20160511
    [9]YANG Hanbing, YANG Wenqiang, SONG Min, ZHANG Jian, ZHANG Xiaoqi, YE Wencai. Phenolic constituents of Galla Chinensis and their antiviral activities[J]. Journal of China Pharmaceutical University, 2016, 47(5): 566-569. DOI: 10.11665/j.issn.1000-5048.20160510
    [10]Isolation and Identification of Two Taxane DiterpenoidCompounds[J]. Journal of China Pharmaceutical University, 2001, (3): 32-34.

Catalog

    Article views (2732) PDF downloads (2608) Cited by()

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return