Research advances in quantitative detection of single cell protein

SHEN Yiyun; QI Xiemin; SONG Qinxin; ZHOU Guohua

doi:10.11665/j.issn.1000-5048.20150502

Journal of China Pharmaceutical University > 2015 > 46(5): 521-531. > DOI: 10.11665/j.issn.1000-5048.20150502

SHEN Yiyun, QI Xiemin, SONG Qinxin, ZHOU Guohua. Research advances in quantitative detection of single cell protein[J]. Journal of China Pharmaceutical University, 2015, 46(5): 521-531. DOI: 10.11665/j.issn.1000-5048.20150502

Citation:

PDF (6314 KB)

Research advances in quantitative detection of single cell protein

More Information

Graphical Abstract

Abstract

Abstract

Proteins presence and differences of the expression level can clarify the physiological or pathological changes in organisms, so the quantitative detection of proteins is vital for disease mechanism research, diagnosis and prognosis evaluation. Traditional protein quantitation methods at the tissue level reflected the average protein expression in cells, but ignore the differences between individual cells. In contrast, approaches for quantitative detection at single-cell level can better reflect the differences. Recently, a number of approaches for such detection have been proposed, including microfluidics, microwell-based technology, optical fiber nanobiosensor, activity-based probe technology and mass spectrometry. The principles, advantages and drawbacks of these approaches are briefly introduced in this review.
- single cell protein,
- quantitative detection,
- research progress,
- microfluidics,
- microwell-based technology,
- optical fiber nanobiosensor,
- probe technology,
- mass spectrometry

FullText(HTML)

References (35)

References

[1]	Cohen D,Dickerson JA,Whitmore CD,et al.Chemical cytometry:fluorescence-based single-cell analysis[J].Annu Rev Anal Chem(Palo Alto Calif),2008,1:165-190.
[2]	Lecault V,Vaninsberghe M,Sekulovic S,et al.High-throughput analysis of single hematopoietic stem cell proliferation in microfluidic cell culture arrays[J].Nat Methods,2011,8(7):581-586.
[3]	Spiller DG,Wood CD,Rand DA,et al.Measurement of single-cell dynamics[J].Nature,2010,465(7299):736-745.
[4]	Zheng XT,Li CM.Single living cell detection of telomerase over-expression for cancer detection by an optical fiber nanobiosensor[J].Biosens Bioelectron,2010,25(6):1548-1552.
[5]	Chattopadhyay PK,Gierahn TM,Roederer M,et al.Single-cell technologies for monitoring immune systems[J].Nat Immunol,2014,15(2):128-135.
[6]	Mu X,Zheng W,Sun J,et al.Microfluidics for manipulating cells[J].Small,2013,9(1):9-21.
[7]	Sims CE,Allbritton NL.Analysis of single mammalian cells on-chip[J].Lab Chip,2007,7(4):423-440.
[8]	Yin H,Marshall D.Microfluidics for single cell analysis[J].Curr Opin Biotechnol,2012,23(1):110-119.
[9]	Borland LM,Kottegoda S,Phillips KS,et al.Chemical analysis of single cells[J].Annu Rev Anal Chem(Palo Alto Calif),2008,1:191-227.
[10]	Whitesides GM.The origins and the future of microfluidics[J].Nature,2006,442(7101):368-373.
[11]	Huang B,Wu H,Bhaya D,et al.Counting low-copy number proteins in a single cell[J].Science,2007,315(5808):81-84.
[12]	Wei W,Shin YS,Ma C,et al.Microchip platforms for multiplex single-cell functional proteomics with applications to immunology and cancer research[J].Genome Med,2013,5(8):75.
[13]	Shin YS,Ahmad H,Shi Q,et al.Chemistries for patterning robust DNA microbarcodes enable multiplex assays of cytoplasm proteins from single cancer cells[J].Chemphyschem,2010,11(14):3063-3069.
[14]	Ma C,Fan R,Ahmad H,et al.A clinical microchip for evaluation of single immune cells reveals high functional heterogeneity in phenotypically similar T cells[J].Nat Med,2011,17(6):738-743.
[15]	Ahmad H,Sutherland A,Shin YS,et al.A robotics platform for automated batch fabrication of high density,microfluidics-based DNA microarrays,with applications to single cell,multiplex assays of secreted proteins[J].Rev Sci Instrum,2011,82(9):094301.
[16]	Shi Q,Qin L,Wei W,et al.Single-cell proteomic chip for profiling intracellular signaling pathways in single tumor cells[J].Proc Natl Acad Sci U S A,2012,109(2):419-424.
[17]	Wang J,Tham D,Wei W,et al.Quantitating cell-cell interaction functions with applications to glioblastoma multiforme cancer cells[J].Nano Lett,2012,12(12):6101-6106.
[18]	Deng Y,Zhang Y,Sun S,et al.An integrated microfluidic chip system for single-cell secretion profiling of rare circulating tumor cells[J].Sci Rep,2014,4:7499.
[19]	Bailey RC,Kwong GA,Radu CG,et al.DNA-encoded antibody libraries:a unified platform for multiplexed cell sorting and detection of genes and proteins[J].J Am Chem Soc,2007,129(7):1959-1967.
[20]	Salehi-Reyhani A,Kaplinsky J,Burgin E,et al.A first step towards practical single cell proteomics:a microfluidic antibody capture chip with TIRF detection[J].Lab Chip,2011,11(7):1256-1261.
[21]	Lu Y,Chen JJ,Mu L,et al.High-throughput secretomic analysis of single cells to assess functional cellular heterogeneity[J].Anal Chem,2013,85(4):2548-2556.
[22]	Walt DR.Protein measurements in microwells[J].Lab Chip,2014,14(17):3195-3200.
[23]	Rissin DM,Kan CW,Campbell TG,et al.Single-molecule enzyme-linked immunosorbent assay detects serum proteins at subfemtomolar concentrations[J].Nat Biotechnol,2010,28(6):595-599.
[24]	Hughes AJ,Spelke DP,Xu Z,et al.Single-cell western blotting[J].Nat Methods,2014,11(7):749-755.
[25]	Ganau M,Bosco A,Palma A,et al.A DNA-based nano-immunoassay for the label-free detection of glial fibrillary acidic protein in multicell lysates[J].Nanomedicine,2015,11(2):293-300.
[26]	Xu F,Zhao H,Feng X,et al.Single-cell chemical proteomics with an activity-based probe:identification of low-copy membrane proteins on primary neurons[J].Angew Chem Int Ed Engl,2014,53(26):6730-6733.
[27]	Norris JL,Caprioli RM.Analysis of tissue specimens by matrix-assisted laser desorption/ionization imaging mass spectrometry in biological and clinical research[J].Chem Rev,2013,113(4):2309-2342.
[28]	Zenobi R.Single-cell metabolomics:analytical and biological perspectives[J].Science,2013,342(6163):1243259.
[29]	Wu M,Singh AK.Single-cell protein analysis[J].Curr Opin Biotechnol,2012,23(1):83-88.
[30]	Mellors JS,Jorabchi K,Smith LM,et al.Integrated microfluidic device for automated single cell analysis using electrophoretic separation and electrospray ionization mass spectrometry[J].Anal Chem,2010,82(3):967-973.
[31]	Bendall SC,Simonds EF,Qiu P,et al.Single-cell mass cytometry of differential immune and drug responses across a human hematopoietic continuum[J].Science,2011,332(6030):687-696.
[32]	Bendall SC,Nolan GP,Roederer M,et al.A deep profiler′s guide to cytometry[J].Trends Immunol,2012,33(7):323-332.
[33]	Ye H,Greer T,Li L.Probing neuropeptide signaling at the organ and cellular domains via imaging mass spectrometry[J].J Proteomics,2012,75(16):5014-5026.
[34]	Delaune A,Cabin-Flaman A,Legent G,et al.50nm-scale localization of single unmodified,isotopically enriched,proteins in cells[J].PLoS One,2013,8(2):e56559.
[35]	Passarelli MK,Ewing AG.Single-cell imaging mass spectrometry[J].Curr Opin Chem Biol,2013,17(5):854-859.

[1]	TIAN Zhixin. Progresses of mass spectrometry-based analysis of N-glycoproteins[J]. Journal of China Pharmaceutical University, 2023, 54(6): 662-673. DOI: 10.11665/j.issn.1000-5048.2023062701
[2]	WANG Maolin, GUO Weiwei, ZHENG Yueqin. Advances in fluorescence probes for detection of hydrogen polysulfides[J]. Journal of China Pharmaceutical University, 2023, 54(5): 553-563. DOI: 10.11665/j.issn.1000-5048.2023042804
[3]	WU Jing, YU Xinyue, XU Yan, HUANG Yin, ZHANG Yuxin. Metabolomics profiling of doxorubicin-induced cardiotoxicity in mice with lung injury[J]. Journal of China Pharmaceutical University, 2023, 54(2): 198-207. DOI: 10.11665/j.issn.1000-5048.20221218001
[4]	YU Lili, CHU Tingting, HOU Chenzhi, WANG Youmei, DI Bin. Quantitative determination of 10 illicit drugs in wastewater by liquid-liquid extraction-ultra performance liquid chromatography-tandem mass spectrometry[J]. Journal of China Pharmaceutical University, 2021, 52(6): 707-712. DOI: 10.11665/j.issn.1000-5048.20210608
[5]	LI Xinxin, WU Huan, WANG Chen, FENG Fang. Mass spectrometry imaging and its application in pharmaceutical sciences[J]. Journal of China Pharmaceutical University, 2014, 45(1): 17-25. DOI: 10.11665/j.issn.1000-5048.20140103
[6]	ZHAO Bo, CHEN Jiang-ning, ZHANG Jun-feng. Copper (II) fluorescent probe for detecting nitric oxide[J]. Journal of China Pharmaceutical University, 2011, 42(6): 490-494.
[7]	Identification of Synthetic Product from p Nitrocinnamaldehyde by Fourier Transform Mass Spectrometry[J]. Journal of China Pharmaceutical University, 1996, (4): 50-52.
[8]	Study on Acyloxy Positions of Angular Diacyloxydihydro pyranocoumarins by Mass Spectrometry[J]. Journal of China Pharmaceutical University, 1996, (3): 10-12.
[9]	Study on the Molecular Weights of Complicated Organic Compounds by Self-Chemical Ionization/Fourier Transform Mass Spectrometry[J]. Journal of China Pharmaceutical University, 1991, (5): 275-278.
[10]	Structure Study on the Dauricine and Daurisoline and Their Derivatives by Self-Chemical Ionization/Fourier Transform Mass Spectrometry[J]. Journal of China Pharmaceutical University, 1991, (1): 5-7.

Cited By

Get Citation

PDF

XML

Article views (1087) PDF downloads (2441)

Turn off MathJax

Article Contents

Abstract

References

Research advances in quantitative detection of single cell protein

Abstract

References

Related Articles

Catalog

Research advances in quantitative detection of single cell protein

Abstract

References

Related Articles

Catalog

Export File

Citation

Format

Content