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ZHAO Zhiying, LYU Huixia, YANG Jun. Preparation of lactoferrin-modified silymarin nanoemulsion and its pharmacokinetics in rats[J]. Journal of China Pharmaceutical University, 2015, 46(6): 665-670. DOI: 10.11665/j.issn.1000-5048.20150605
Citation: ZHAO Zhiying, LYU Huixia, YANG Jun. Preparation of lactoferrin-modified silymarin nanoemulsion and its pharmacokinetics in rats[J]. Journal of China Pharmaceutical University, 2015, 46(6): 665-670. DOI: 10.11665/j.issn.1000-5048.20150605
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Preparation of lactoferrin-modified silymarin nanoemulsion and its pharmacokinetics in rats

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  • In this paper, silymarin nanoemulsions(SM-NE)were prepared based upon PC/Tween 80 system, and adsorption of lactoferrin(LF)absorbed onto the surface of silymarin nanoemulsions via electrostatic interaction resulted in the formation of lactoferrin-modified silybin nanoemulsions(LF-SM-NE). SM-NE and LF-SM-NE were characterized in terms of the shape, diameter, zeta potential, and entrapment efficiency. In addition, in vivo absorption of SM-NE and LF-SM-NE following oral dosing was investigated by the application of LC-MS/MS in the determination of silymarin in blood sample. The results showed that the saturated adsorption of Lf at the surface of the SM-NE occurred at LF concentration of 0. 15 mg/mL and the incubation time of 4 h. LF-SM-NE were spherical with average particle size of(257±5. 9)nm, zeta potential of -(33. 1±1. 5)mV, entrapment efficiency(96. 9±1. 9)% and drug loading(3. 90±0. 12)%. Compared with those of SM-NE, LF-SM-NE significantly increased drug levels of LF in rats were observed after dosing of LF-SM-NE, and both of cmax and AUC were increased by approximately 2-fold. In summary, LF-SM-NE could improve oral drug absorption of LF in rats. The approach of enhancement in oral absorption of the nanoemulsions shows a promise for the application.
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  • [1]
    Liu ZG,Li XL,Weng LD,et al.Research progress in pharmacological effects of silymarin[J].J Liaoning Univ TCM(辽宁中医药大学学报),2012,14(10):91-93.
    [2]
    Zhuang SS,Chen JM,Wang WY,et al.Research on improving bioavailability of silymarin by new technology[J].Chin J Hosp Pharm(中国医院药学杂志),2014,34(24):2158-2161.
    [3]
    Parveen R,Baboota S,Ali J,et al.Effects of silymarin nanoemulsion against carbon tetrachloride-induced hepatic damage[J].Arch Pharm Res,2011,34(5):767-774.
    [4]
    Yuan H.Study on gastrointestinal transport of solid lipid nanoparticles by oral drug carrier(固体脂质纳米粒口服药物载体经胃肠道转运的研究)[D].Hangzhou:Zhejiang University,2008.
    [5]
    Shofner JP,Phillips MA,Peppas NA.Cellular evaluation of synthesized insulin/transferrin bioconjugates for oral insulin delivery using intelligent complexation hydrogels[J].Macromol Biosci,2010,10(3):299-306.
    [6]
    Amet N,Wang W,Shen WC.Human growth hormone-transferrin fusion protein for oral delivery in hypophysectomized rats[J].J Control Release,2010,141(20):177-182.
    [7]
    Chinese Pharmacopeia Commission.WS-10001-(HD-0600)-2002,National Drug Standards for Chemicals Promoted from Local Standards.Vol 7(国家药品标准:化学药品地方标准上升国家标准.第七册)[S].Beijing:China Medical Science Press,2002:30-31.
    [8]
    Luo XJ,Li W,Han JP,et al.HPLC-MS/MS study on pharmacokinetics of silybin A and silybin B in rat plasma[J].Chin J Pharm Anal(药物分析杂志),2009,29(12):2002-2006.
    [9]
    Chen HL,Wu MJ,Wang X,et al.Construction and characterization of lactoferrin modified procationic liposomes[J].Central South Pharm(中南药学),2010,8(2):81-84.
    [10]
    Xiao YY,Chen X,Zou L,et al.Preparation of lactoferrin-modified nanostructured lipid carriers and evaluation of its brain targeting efficiency[J].Chin Pharm J(中国药学杂志),2013,48(20),1755-1760.
    [11]
    Yang J,Xie J.Determination of lyso-phosphatidylcholine and lyso-phosphatidylethanolamine in docetaxel concentrated Solution for Injection[J].Chin J Mod Appl Pharm(中国现代应用药学),2013,30(11):1210-1214.
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