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CHEN Jiaquan, WANG Hui, ZHANG Zhiyuan, ZHAI Xiaoting, YIN Rong, ZHU Fenxia. Preparation of pumiloside and identification of its metabolites in rats by UPLC-QTOF/MS[J]. Journal of China Pharmaceutical University, 2015, 46(6): 677-682. DOI: 10.11665/j.issn.1000-5048.20150607
Citation: CHEN Jiaquan, WANG Hui, ZHANG Zhiyuan, ZHAI Xiaoting, YIN Rong, ZHU Fenxia. Preparation of pumiloside and identification of its metabolites in rats by UPLC-QTOF/MS[J]. Journal of China Pharmaceutical University, 2015, 46(6): 677-682. DOI: 10.11665/j.issn.1000-5048.20150607
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Preparation of pumiloside and identification of its metabolites in rats by UPLC-QTOF/MS

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  • In this paper, water extract of Nauclea officinalis was absorbed by AB-8 macroporous resin and subsequently gradient-eluted with alcoholic solution of different proportion to prepare pumiloside monomer of high purity. The metabolites in urine, feces and bile of rats with gavage administration were analyzed by UPLC-QTOF/MS. Accurate MS/MS data of all components were collected with full scan mode, and analyzed by MetaboLynx software. Results showed that the monomer of high-purity pumiloside was prepared and four metabolites in rats were identified.
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