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ZHANG Lu, PAN Peipei, CHEN Saizhen. Determination of catechin, epicatechin, gallic acid and procyanidin B2 in procyanidins by RP-HPLC[J]. Journal of China Pharmaceutical University, 2016, 47(1): 54-57. DOI: 10.11665/j.issn.1000-5048.20160107
Citation: ZHANG Lu, PAN Peipei, CHEN Saizhen. Determination of catechin, epicatechin, gallic acid and procyanidin B2 in procyanidins by RP-HPLC[J]. Journal of China Pharmaceutical University, 2016, 47(1): 54-57. DOI: 10.11665/j.issn.1000-5048.20160107
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Determination of catechin, epicatechin, gallic acid and procyanidin B2 in procyanidins by RP-HPLC

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  • To determine catechin(C), epicatechin(EC), gallic acid(GA)and procyanidin B2(PCB2)in procyanidins and compare their contents in procyanidins from different manufacturers, an A RP-HPLC method was developed. The determination was carried out on a Hypersil ODS2 column(4. 0 mm×200 mm, 5 μm). The mobile phase consisted of methanol and 2% acetic acid with gradient elution and the detection wave-length at 280 nm. There was a good linear relationship between concentration and the peak area in the range of 0. 1-50 μg/mL(r=0. 998 6)for catechin, 0. 1-50 μg/mL(r=0. 994 5)for epicatechin, 0. 05-50 μg/mL(r=0. 999 9)for gallic acid and 0. 1-50 μg/mL(r=0. 992 2)for procyanidin B2, respectively. The average recoveries of catechin, epicatechin, gallic acid and PCB2 were 98. 36%, 98. 21%, 89. 60% and 98. 47%, respectively and the RSDs were 1. 39%, 0. 84%, 2. 12% and 2. 46%, respectively. The method is simple, accurate, reproducible and can be used for assay of C, EC, GA, PCB2 in procyanidins. There was a great difference in the content of four substance in procyanidins from different manufacturers.
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  • [1]
    Kong JM,Chia LS,Goh NK,et al.Analysis and biological activities of anthocyanins[J].Phytochemistry,2003,64(5):923-933.
    [2]
    Bilia AR,Morelli I,Hamburger M,et al.A-type proanthocyanidins from Prunus prostrat[J].Phytochemistry,1996,43(4):887-892.
    [3]
    Zhang XJ,Xia CT,Wu JM,et al.The research of proanthocyanidin resources[J].J Chin Med Mater(中药材),2009,32(7):1154-1160.
    [4]
    Bao JZ,Chen YK,Xu GH.Primary study on the determination methods of proanthocyanidin content of grape seed extract[J].J Agri Sci(农业科学研究),2005,26(1):43-45.
    [5]
    Zheng C,Li L,Pang H,et al.Glycosylation modification on the natural active components[J].J Cellulose Sci Technol(纤维素科学与技术),2012,20(1):62-71.
    [6]
    Li XL,Cui L,Zhu DQ.Research progress of the biological effects of the gallic acid[J].China Pharm(中国药师),2004,7(10):767-769.
    [7]
    Butterweck V, Liefländer-Wulf U, Winterhoff H, et al. Plasma levels of hypericin in presence of procyanidin B2 and hyperoside:a pharmacokinetic study in rats[J].Planta Med,2003,69(3):189-192.
    [8]
    Lou H,Yamazaki Y,Sasaki T,et al.A-type proanthocyanidins from peanut skins[J].Phytochemistry,1999,51(2):297-308.
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