Effect of block of AGEs-RAGE pathway on the migration of VSMCs

XIANG Min; ZHANG Yaqin; WU Pingping; GAO Zhenyu

doi:10.11665/j.issn.1000-5048.20160212

Journal of China Pharmaceutical University > 2016 > 47(2): 199-203. > DOI: 10.11665/j.issn.1000-5048.20160212

XIANG Min, ZHANG Yaqin, WU Pingping, GAO Zhenyu. Effect of block of AGEs-RAGE pathway on the migration of VSMCs[J]. Journal of China Pharmaceutical University, 2016, 47(2): 199-203. DOI: 10.11665/j.issn.1000-5048.20160212

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Effect of block of AGEs-RAGE pathway on the migration of VSMCs

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To investigate the effect of block of AGEs-RAGE pathway on the migration of aortic vascular smooth muscle in diabetic rats and its possible mechanisms, vascular smooth muscle cells(VSMCs)cells were pre-stimulated by antibody of RAGE, and then stimulated by AGEs. Transwell assay was adopted to assay migration of VSMCs. Proliferation of VSMCs and expression of p27 were analyzed by MTT and ELISA, respectively. The change of ROS level in VSMCs was defermined by DCFH assay, the expression of NOX1 mRNA was determined by RT-PCR assay. Results indicated that the AGEs induction for migration of VSMCs was significantly inhibited after treatment by RAGE antibody(P< 0. 01), which blocked the AGEs-RAGE pathway, and the inhibition of migration was stronger than that of proliferation. The ROS level was decreased(P< 0. 01), and the expression of NOX1 mRNA was decreased, yet the expression of P27 protein was not changed greatly. Block of AGEs-RAGE pathway by antibody of RAGE can inhibit the migration of VSMCs, and the mechanism may be related with the decrease of NOX1 mRNA and then down to the level of intracellular oxidative stress in VSMCs.
- advanced glycation endproducts,
- receptor for advanced glycation endproducts,
- diabetes mellitus,
- VSMCs,
- migration,
- target,
- signal transduction

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